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KGF Antibody (YA323)(PBS only)

(Synonyms: Fgf7; Fibroblast growth factor 7; HBGF7; Heparin binding growth factor 7; Keratinocyte growth factor)

KGF Antibody (YA323) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KGF.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KGF Antibody (YA323)(PBS only)
  • KGF Antibody (YA323)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

KGF Antibody (YA323) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KGF.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Immunogen

Synthetic peptide corresponding to Human KGF aa46-60.

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KGF Antibody (YA323)(PBS only)
    Western blot analysis of extracts from C6 (lane 2(20μg), Hela (lane 3(20μg) and NIH3T3 (lane 4(20μg) using KGF (HY-P80733) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    KGF plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Epithelial cell

  • Isoforms & Post-Translational Modification

    P21781 has 2 isomers: P21781-1: 22509 Da (predicted); P21781-2: 11520 Da (predicted).

  • Subunit

    Interacts with FGFBP1. Interacts with FGFR2. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors

  • SwissProt ID

    P21781

  • Gene ID
    2252 [NCBI]
  • Synonyms

    Fgf7; Fibroblast growth factor 7; HBGF7; Heparin binding growth factor 7; Keratinocyte growth factor

  • Research Field

    Cardiovascular

KGF Antibody (YA323)(PBS only) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), Hela (lane 3(20μg) and NIH3T3 (lane 4(20μg) using KGF (HY-P80733) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • KGF Antibody (YA323)(PBS only) Product Image
    1/16
  • Experimental Validation Results for KGF Antibody (YA323)(PBS only)
  • KGF Antibody (YA323)(PBS only) Product Image
  • Experimental Validation Results for KGF Antibody (YA323)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), Hela (lane 3(20μg) and NIH3T3 (lane 4(20μg) using KGF (HY-P80733) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for KGF Antibody (YA323)(PBS only)
  • KGF Antibody (YA323)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6 (lane 2(20μg), Hela (lane 3(20μg) and NIH3T3 (lane 4(20μg) using KGF (HY-P80733) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • KGF Antibody (YA323)(PBS only) Product Image WB