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KGF Antibody (YA323)

(Synonyms: Fgf7; Fibroblast growth factor 7; HBGF7; Heparin binding growth factor 7; Keratinocyte growth factor)
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Based on 1 Customer Validation

KGF Antibody (YA323) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KGF.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KGF Antibody (YA323)
  • KGF Antibody (YA323) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

KGF Antibody (YA323) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KGF.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human KGF aa46-60.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102682

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KGF Antibody (YA323)
    Western blot analysis of extracts from C6 (lane 2(20μg), Hela (lane 3(20μg) and NIH3T3 (lane 4(20μg) using KGF (HY-P80733) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Epithelial cell

  • Isoforms & Post-Translational Modification

    P21781 has 2 isomers: P21781-1: 22509 Da (predicted); P21781-2: 11520 Da (predicted).

  • Subunit

    Interacts with FGFBP1. Interacts with FGFR2. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors

  • SwissProt ID

    P21781

  • Gene ID
    2252 [NCBI]
  • Synonyms

    Fgf7; Fibroblast growth factor 7; HBGF7; Heparin binding growth factor 7; Keratinocyte growth factor

  • Research Field

    Cardiovascular

KGF Antibody (YA323) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), Hela (lane 3(20μg) and NIH3T3 (lane 4(20μg) using KGF (HY-P80733) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • KGF Antibody (YA323) Product Image
    1/16
  • Experimental Validation Results for KGF Antibody (YA323)
  • KGF Antibody (YA323) Product Image
  • Experimental Validation Results for KGF Antibody (YA323)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), Hela (lane 3(20μg) and NIH3T3 (lane 4(20μg) using KGF (HY-P80733) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for KGF Antibody (YA323)
  • KGF Antibody (YA323) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6 (lane 2(20μg), Hela (lane 3(20μg) and NIH3T3 (lane 4(20μg) using KGF (HY-P80733) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • KGF Antibody (YA323) Product Image WB