IL-1 beta Antibody (YA345)
(Synonyms: IL-1; IL1F2; IL1beta; IL1-BETA)Based on 1 Customer Validation
IL-1 beta Antibody (YA345) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-1 beta.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IF-Tissue
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Reactivity :
Human
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Formulation:
Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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| Dilution Ratio | 1:5000 | 1:50-1:200 | 1:50-1:200 | 1:50-1:200 |
Product Details
IL-1 beta Antibody (YA345) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-1 beta.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 17 kDaNote: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 31 kDa
Entrez Gene: 3553 Human ; 16176 Mouse
SwissProt: P01584 Human ; P10749 Mouse ; Q63264 Rat
OMIM: 147720 Human
Synthetic peptide within C-terminal human IL1 beta.
Endogenous
Protein A affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from THP-1 (lane 1(20μg)) 、THP-1+LPS (lane 2(20μg)) 、Raw 264.7 (lane 3(20μg)) 、Raw 264.7+LPS (lane 4(20μg)) 、A431 (lane 5(20μg)) 、A549 (lane 6(20μg)) 、Mouse spleen (lane 7(20μg)) and Mouse lung (lane 8(20μg)) using IL-1 beta Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80503, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
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Western blot analysis of extracts from THP-1 (lane 2(20μg) and THP-1 (lane 3(20μg) , LPS treated (HY-D1056, 100ng/mL, 2hrs) as indicated, using IL-1 beta(HY-P80503) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
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Western blot analysis of extracts from THP-1 (lane 1(20μg)) 、THP-1+LPS (lane 2(20μg)) 、Raw 264.7 (lane 3(20μg)) 、Raw 264.7+LPS (lane 4(20μg)) 、A431 (lane 5(20μg)) 、A549 (lane 6(20μg)) 、Mouse spleen (lane 7(20μg)) and Mouse lung (lane 8(20μg)) using IL-1 beta Antibody (HY-P80503) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using IL-1 Beta Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80503, 1/100) , competitor's antibody (1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using IL-1 beta Antibody (YA345). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80503, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using IL-1 beta Antibody (YA345). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80503, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Background
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Function
Interleukin-1β (IL-1β) is one of the pro-inflammatory cytokines and is produced and secreted by a variety of cell types although the vast majority of studies have focussed on its production within cells of the innate immune system, such as monocytes and macrophages. IL-1β is produced as inactive pro-IL-1β (encoded by pro-Il-1b) in response to inflammatory stimuli, including both microbial products and endogenous danger-associated molecules. IL-1β could regulate the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. IL-1β also plays a significant regulator of reproduction in females[1][2].
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Subcellular Localization
Cytoplasm, cytosol; Secreted; Lysosome; Secreted, extracellular exosome
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Expression
Tissue_specificity:Expression (protein level) in activated monocytes/macrophages.
Induction:By LPS (PubMed:15192144) . Transcription and translation induced by M.tuberculosis and a number of different M.tuberculosis components in macrophages; EsxA is the most potent activator tested (at protein level) (PubMed:20148899) . In pancreatic islets, release is increased by high glucose treatment. In pancreatic islets and macrophages, release is also increased by endocannabinoid anandamide/AEA (PubMed:23955712) -
Isoforms & Post-Translational Modification
1) IL1beta (P01584): Number of amino acids: 269; Molecular weight (Da): 30,748
2) Mouse IL1beta (P10749): Number of amino acids: 269; Molecular weight (Da): 30,931
3) Rat IL1beta (a63264): Number of amino acids: 268; Molecular weight (Da): 30,644 -
Subunit
Monomer. In its precursor form, weakly interacts with full-length MEFV; the mature cytokine does not interact at all (PubMed:17431422). Interacts with integrins ITGAV:ITGBV and ITGA5:ITGB1; integrin-binding is required for IL1B signaling (PubMed:29030430). Interacts with cargo receptor TMED10; the interaction is direct and is required for the secretion of IL1B mature form (PubMed:32272059). Interacts with HSP90AB1; the interaction facilitates cargo translocation into the ERGIC (PubMed:32272059). Interacts with HSP90B1; the interaction facilitates cargo translocation into the ERGIC (PubMed:32272059)
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SwissProt ID
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Synonyms
IL-1; IL1F2; IL1beta; IL1-BETA
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Research Field
Immunology
Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
[1]. Jan Petrasek, et al. IL-1 receptor antagonist ameliorates inflammasome-dependent alcoholic steatohepatitis in mice. J Clin Invest. 2012 Oct;122(10):3476-89. [Content Brief]
[2]. Karina Zitta, et al. Interleukin-1beta regulates cell proliferation and activity of extracellular matrix remodelling enzymes in cultured primary pig heart cells. Biochem Biophys Res Commun. 2010 Sep 3;399(4):542-7. [Content Brief]