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CD133 Antibody (YA808)

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Based on 3 publication(s) in Google Scholar

CD133 Antibody (YA808) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD133.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD133 Antibody (YA808)
  • CD133 Antibody (YA808) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500 1:600 1:500-1:1000

Product Details

Description

CD133 Antibody (YA808) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD133.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 120 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 97 kDa
Species Reactivity Database

Entrez Gene: 8842 Human

SwissProt: O43490 Human

Immunogen

Synthetic peptide corresponding to Human CD133.AA range:151-400.

Sensitivity

Endogenous

Purification

Protein G affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3102063

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD133 Antibody (YA808)
    Western blot analysis of extracts from HT-29 (lane 2(20μg) and HT-29 (lane 3(40μg)using CD133 (HY-P80054) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80054, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439)

  • Subcellular Localization

    Apical cell membrane; Multi-pass membrane protein; Cell projection, microvillus membrane; Multi-pass membrane protein; Cell projection, cilium, photoreceptor outer segment; Endoplasmic reticulum; Endoplasmic reticulum-Golgi intermediate compartment

  • Expression


    Tissue_specificity:Isomer 1 is selectively expressed on CD34+ hematopoietic stem cells and progenitor cells in human bone marrow, fetal liver, umbilical cord blood, and human peripheral blood. Isomer 1 is not detected in other blood cells. Isomer 1 is also expressed in a variety of non-lymphoid tissues, including the retina, pancreas, placenta, kidney, liver, lung, brain, and heart. In saliva, isomer 1 exists as small membrane granules. Isomer 2 is primarily expressed in fetal liver, skeletal muscle, kidney, and heart, as well as in human pancreas, kidney, liver, lung, and placenta. Isomer 2 is highly expressed in fetal liver, lowly expressed in bone marrow, and almost undetectable in peripheral blood. Isomer 2 is expressed on hematopoietic stem cells and epidermal basal cells (protein level) . In the human retina, isomer 2 is expressed by rod cells and cone cells (protein level) .

  • Isoforms & Post-Translational Modification

    O43490 has 7 isomers: O43490-1: 97202 Da (predicted); O43490-2: 96251 Da (predicted); O43490-3: 93270 Da (predicted); O43490-4: 93654 Da (predicted); O43490-5: 92741 Da (predicted); O43490-6: 93692 Da (predicted); O43490-7: 94605 Da (predicted).
    Isoform 1 and isoform 2 are glycosylated;Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis

  • Subunit

    Interacts with CDHR1 and with actin filaments. Interacts with NAT8 and NAT8B

  • SwissProt ID

    O43490

  • Gene ID
    8842 [NCBI]
  • Research Field

    Neuroscience

CD133 Antibody (YA808) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HT-29 (lane 2(20μg) and HT-29 (lane 3(40μg)using CD133 (HY-P80054) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80054, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD133 Antibody (YA808) Product Image
    1/16
  • Experimental Validation Results for CD133 Antibody (YA808)
  • CD133 Antibody (YA808) Product Image
  • Experimental Validation Results for CD133 Antibody (YA808)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HT-29 (lane 2(20μg) and HT-29 (lane 3(40μg)using CD133 (HY-P80054) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80054, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD133 Antibody (YA808)
  • CD133 Antibody (YA808) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HT-29 (lane 2(20μg) and HT-29 (lane 3(40μg)using CD133 (HY-P80054) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80054, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD133 Antibody (YA808) Product Image WB