Copy Link Print Information

EAAT1 Antibody (YA2479)

(Synonyms: SLC1A3; EAAT1; GLAST; GLAST1; Excitatory amino acid transporter 1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3)
Customer Review

Based on 1 Customer Validation

EAAT1 Antibody (YA2479) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EAAT1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for EAAT1 Antibody (YA2479)
  • EAAT1 Antibody (YA2479) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

EAAT1 Antibody (YA2479) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EAAT1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 60 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human EAAT1 aa170-222.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104832

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EAAT1 Antibody (YA2479)
    Western blot analysis of extracts from A431(lane 2(20ug) and A431(lane 3(40ug) using EAAT1 Antibody (HY-P82734) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    The excitatory amino acid transporter 1 (EAAT1, also known as GLAST or SLC1A3) is a high-affinity Na+-dependent glutamate transporter primarily expressed in astrocytes, where it regulates extracellular glutamate concentrations and prevents excitotoxic neuronal damage[1][2][3]. Mechanistically, EAAT1 mediates glutamate uptake by coupling the transport of glutamate with Na+ and K+ ions, enabling clearance against steep concentration gradients and maintaining synaptic homeostasis[3][4]. Compared with EAAT2, EAAT1 exhibits a more restricted spatial distribution, with higher expression in spinal cord laminae 1-3 and in cerebellar glial populations, and demonstrates a unique substrate- and Na+-independent leak conductance that distinguishes it from neuronal isoforms EAAT3-EAAT5[5][4]. In disease models, EAAT1 dysregulation is implicated in neurodegenerative disorders, psychiatric conditions, and platelet dysfunction, indicating its role in both central and peripheral glutamate handling[6][7][8]. Recent studies reveal that EAAT1 expression is crucial for the proliferation and survival of T-cell acute lymphoblastic leukemia (T-ALL), where it supports glutamine-derived aspartate synthesis and nucleotide production, highlighting its potential as a therapeutic target[9]. Pharmacological inhibition using selective antagonists, such as UCPH101, demonstrates EAAT1’s dominant role in glutamate clearance at glial synapses, in contrast to other EAAT subtypes which show secondary or context-dependent activity[10].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Detected in brain tissue (PubMed:7521911, PubMed:8123008, PubMed:8218410) . Extremely low levels in the heart, lungs, placenta, and skeletal muscle (PubMed:7521911, PubMed:8123008) . Highly expressed in the cerebellum, but also present in the frontal cortex, hippocampus, and basal ganglia (PubMed:7521911) .

  • Isoforms & Post-Translational Modification

    P43003 has 2 isomers: P43003-1: 59572 Da (predicted); P43003-2: 55149 Da (predicted).
    Glycosylated

  • Subunit

    Homotrimer (PubMed:28424515)

  • SwissProt ID

    P43003

  • Gene ID
    6507 [NCBI]
  • Synonyms

    SLC1A3; EAAT1; GLAST; GLAST1; Excitatory amino acid transporter 1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3

  • Research Field

    Neuroscience

References

[1]. Zhou Y, et al. GABA and Glutamate Transporters in Brain. Front Endocrinol (Lausanne). 2013;4:165. [Content Brief]

[2]. Parkin GM, et al. Glutamate transporters, EAAT1 and EAAT2, are potentially important in the pathophysiology and treatment of schizophrenia and affective disorders. World J Psychiatry. 2018 Jun 28;8(2):51-63. [Content Brief]

[3]. Rauen T, et al. Structural and functional dynamics of Excitatory Amino Acid Transporters (EAAT). AIMS Molecular Science. 2014;1(3):99-125.

[4]. Divito CB, et al. Glial and Neuronal Glutamate Transporters Differ in the Na+ Requirements for Activation of the Substrate-Independent Anion Conductance. Front Mol Neurosci. 2017 May 29;10:150. [Content Brief]

[5]. Hu QX, et al. Semi-quantitative distribution of excitatory amino acid (glutamate) transporters 1-3 (EAAT1-3) and the cystine-glutamate exchanger (xCT) in the adult murine spinal cord. Neurochem Int. 2020 Nov;140:104811. [Content Brief]

[6]. Zoia C, et al. Glutamate transporters in platelets: EAAT1 decrease in aging and in Alzheimer's disease. Neurobiol Aging. 2004 Feb;25(2):149-57. [Content Brief]

[7]. Karki P, et al. Genetic dys-regulation of astrocytic glutamate transporter EAAT2 and its implications in neurological disorders and manganese toxicity. Neurochem Res. 2015 Feb;40(2):380-8. [Content Brief]

[8]. Bauer D, et al. Abnormal glycosylation of EAAT1 and EAAT2 in prefrontal cortex of elderly patients with schizophrenia. Schizophr Res. 2010 Mar;117(1):92-8. [Content Brief]

[9]. Stanulović VS, et al. The glutamate/aspartate transporter EAAT1 is crucial for T-cell acute lymphoblastic leukemia proliferation and survival. Haematologica. 2024 Nov 1;109(11):3505-3519. [Content Brief]

[10]. Tse DY, et al. Pharmacological inhibitions of glutamate transporters EAAT1 and EAAT2 compromise glutamate transport in photoreceptor to ON-bipolar cell synapses. Vision Res. 2014 Oct;103:49-62. [Content Brief]

View More

EAAT1 Antibody (YA2479) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431(lane 2(20ug) and A431(lane 3(40ug) using EAAT1 Antibody (HY-P82734) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • EAAT1 Antibody (YA2479) Product Image
    1/16
  • Experimental Validation Results for EAAT1 Antibody (YA2479)
  • EAAT1 Antibody (YA2479) Product Image
  • Experimental Validation Results for EAAT1 Antibody (YA2479)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431(lane 2(20ug) and A431(lane 3(40ug) using EAAT1 Antibody (HY-P82734) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EAAT1 Antibody (YA2479)
  • EAAT1 Antibody (YA2479) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431(lane 2(20ug) and A431(lane 3(40ug) using EAAT1 Antibody (HY-P82734) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • EAAT1 Antibody (YA2479) Product Image WB