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DNA Polymerase beta Antibody (YA3378)

(Synonyms: POLB; DNA polymerase beta)
Customer Review

Based on 1 Customer Validation

DNA Polymerase beta Antibody (YA3378) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DNA Polymerase beta.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
  • DNA Polymerase beta Antibody (YA3378) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50

Product Details

Description

DNA Polymerase beta Antibody (YA3378) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DNA Polymerase beta.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 38 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 38 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human DNA Polymerase beta aa300-335/335.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105740

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
    Western blot analysis of extracts from A431 (lane 2(20μg), A431 (lane 3(40μg), using DNA Polymerase beta Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
    Immunocytochemistry analysis of Hela cells labeling DNA Polymerase beta with DNA Polymerase beta Antibody (HY-P83633) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DNA Polymerase beta Antibody (HY-P83633) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
    Immunocytochemistry analysis of A549 cells labeling DNA Polymerase beta with DNA Polymerase beta Antibody (HY-P83633) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DNA Polymerase beta Antibody (HY-P83633)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    DNA Polymerase beta is a Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase[1][2][3][4][5][6].

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Subunit

    Monomer. Binds single-stranded DNA (ssDNA) (PubMed:9556598). Interacts with APEX1, LIG1, LIG3, FEN1, PCNA and XRCC1 (PubMed:19336415, PubMed:26760506, PubMed:9207062). Interacts with HUWE1/ARF-BP1, STUB1/CHIP and USP47. Interacts with FAM168A (PubMed:19336415, PubMed:25260657, PubMed:26760506)

  • SwissProt ID

    P06746

  • Gene ID
    5423 [NCBI]
  • Synonyms

    POLB; DNA polymerase beta

  • Research Field

    Epigenetics and Nuclear Signaling

DNA Polymerase beta Antibody (YA3378) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from A431 (lane 2(20μg), A431 (lane 3(40μg), using DNA Polymerase beta Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling DNA Polymerase beta with DNA Polymerase beta Antibody (HY-P83633) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DNA Polymerase beta Antibody (HY-P83633) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling DNA Polymerase beta with DNA Polymerase beta Antibody (HY-P83633) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DNA Polymerase beta Antibody (HY-P83633)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • DNA Polymerase beta Antibody (YA3378) Product Image
    1/16
  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
  • DNA Polymerase beta Antibody (YA3378) Product Image
  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from A431 (lane 2(20μg), A431 (lane 3(40μg), using DNA Polymerase beta Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling DNA Polymerase beta with DNA Polymerase beta Antibody (HY-P83633) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DNA Polymerase beta Antibody (HY-P83633) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling DNA Polymerase beta with DNA Polymerase beta Antibody (HY-P83633) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DNA Polymerase beta Antibody (HY-P83633)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
  • Experimental Validation Results for DNA Polymerase beta Antibody (YA3378)
  • DNA Polymerase beta Antibody (YA3378) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from A431 (lane 2(20μg), A431 (lane 3(40μg), using DNA Polymerase beta Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • DNA Polymerase beta Antibody (YA3378) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling DNA Polymerase beta with DNA Polymerase beta Antibody (HY-P83633) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DNA Polymerase beta Antibody (HY-P83633) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • DNA Polymerase beta Antibody (YA3378) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling DNA Polymerase beta with DNA Polymerase beta Antibody (HY-P83633) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DNA Polymerase beta Antibody (HY-P83633)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • DNA Polymerase beta Antibody (YA3378) Product Image WB
  • DNA Polymerase beta Antibody (YA3378) Product Image ICC
  • DNA Polymerase beta Antibody (YA3378) Product Image ICC