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VAMP2 Antibody (YA1418)

(Synonyms: vesicle-associated membrane protein 2 (synaptobrevin 2); SYB2; VAMP-2)
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Based on 1 Customer Validation

VAMP2 Antibody (YA1418) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VAMP2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for VAMP2 Antibody (YA1418)
  • Experimental Validation Results for VAMP2 Antibody (YA1418)
  • VAMP2 Antibody (YA1418) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

VAMP2 Antibody (YA1418) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VAMP2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 13 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 13 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human VAMP2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103782

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for VAMP2 Antibody (YA1418)
    Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), and Rat brain (lane 2, 15 μg) using VAMP2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for VAMP2 Antibody (YA1418)
    Western blot analysis of extracts from C6(lane 2(20ug) and C6(lane 3(40ug) using VAMP2 Antibody (HY-P81041) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    VAMP2 is involved in the targeting and/or fusion of transport vesicles to their target membrane. Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1[1].

  • Subcellular Localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein; Cell membrane

  • Expression


    Tissue_specificity:Nervous system and skeletal muscle

  • Subunit

    Interacts with VAPA and VAPB. Interacts with WDFY2, PRKCZ and PRKCI (PubMed:17313651). Forms a complex with WDFY2 and PRKCZ (PubMed:17313651).

  • SwissProt ID

    P63027

  • Gene ID
    6844 [NCBI]
  • Synonyms

    vesicle-associated membrane protein 2 (synaptobrevin 2); SYB2; VAMP-2

  • Research Field

    Signal Transduction

References

[1]. Salpietro V, et al. Mutations in the Neuronal Vesicular SNARE VAMP2 Affect Synaptic Membrane Fusion and Impair Human Neurodevelopment. Am J Hum Genet. 2019 Apr 4;104(4):721-730. [Content Brief]

VAMP2 Antibody (YA1418) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), and Rat brain (lane 2, 15 μg) using VAMP2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug) and C6(lane 3(40ug) using VAMP2 Antibody (HY-P81041) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • VAMP2 Antibody (YA1418) Product Image
    1/16
  • Experimental Validation Results for VAMP2 Antibody (YA1418)
  • Experimental Validation Results for VAMP2 Antibody (YA1418)
  • VAMP2 Antibody (YA1418) Product Image
  • Experimental Validation Results for VAMP2 Antibody (YA1418)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), and Rat brain (lane 2, 15 μg) using VAMP2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for VAMP2 Antibody (YA1418)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug) and C6(lane 3(40ug) using VAMP2 Antibody (HY-P81041) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for VAMP2 Antibody (YA1418)
  • Experimental Validation Results for VAMP2 Antibody (YA1418)
  • VAMP2 Antibody (YA1418) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), and Rat brain (lane 2, 15 μg) using VAMP2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • VAMP2 Antibody (YA1418) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6(lane 2(20ug) and C6(lane 3(40ug) using VAMP2 Antibody (HY-P81041) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • VAMP2 Antibody (YA1418) Product Image WB
  • VAMP2 Antibody (YA1418) Product Image WB