POU3F2 Antibody (YA3865)(PBS only)

Cat. No.: HY-P84168A: Data Sheet User Guide for Antibodies
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POU3F2 Antibody (YA3865) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to POU3F2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

POU3F2 Antibody (YA3865) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to POU3F2.

Host

Mouse

Molecular Weight

Predicted band size: 47 kDa;

Observed band size: 47 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P20265

Gene ID

5454 [NCBI]

Immunogen

Purified recombinant fragment of human POU3F2 (AA: 214-362) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:250
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Specifically expressed in neuroectodermal cell lineages

Subunit：Interacts with PQBP1 (PubMed:10332029). Interaction with ISL1 (By similarity)

Synonyms

BRN2; OCT7; OTF7; OTF-7; POUF3; brn-2; oct-7; N-Oct3

Documentation

User Guide for Antibodies (1077 KB)