PIP4K2A Antibody (YA8512)
(Synonyms: PI5P4KA; PIP5K2A; PIP5KII-alpha; PIP5KIIA; PIPK)PIP4K2A Antibody (YA8512) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PIP4K2A.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
|
|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-250 | 1:100 |
Product Details
PIP4K2A Antibody (YA8512) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PIP4K2A.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 46 kDa
Full length human recombinant protein of human PIP4K2A produced in HEK293T cell.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PIP4K2A encodes phosphatidylinositol-5-phosphate 4-kinase α (PI5P4Kα), a type II phosphoinositide kinase that phosphorylates phosphatidylinositol-5-phosphate (PI5P) to generate phosphatidylinositol-4, 5-bisphosphate (PI (4, 5) P2), a key signaling lipid that supports phosphoinositide-dependent cellular regulation and membrane-associated signaling processes[1][2]. Mechanistically, PI5P4Kα contributes to intracellular phosphoinositide homeostasis and has been linked to autophagy regulation, nutrient-stress responses, and metabolic control through coordination of organelle-associated signaling networks[3][4]. These functions have attracted substantial interest in cancer biology, where genetic or pharmacological disruption of PI5P4K family members has been associated with altered cellular metabolism, impaired autophagic flux, and reduced proliferation in selected tumor models, particularly those with defects in TP53 signaling[5][6]. Compared with related isoforms, PIP4K2A, PIP4K2B, and PIP4K2C encode PI5P4Kα, PI5P4Kβ, and PI5P4Kγ, respectively, but the isoforms display distinct enzymatic activities, with PI5P4Kα exhibiting the highest catalytic activity and PI5P4Kγ showing markedly lower activity in biochemical assays[7]. For experimental applications, PI5P4Kα has become a tractable pharmacological target, and both the covalent pan-PI5P4K inhibitor THZ-P1-2 and the highly selective PIP4K2A inhibitors BAY-091 and BAY-297 are widely used as chemical probes to investigate PI5P4K signaling, autophagy, cancer metabolism, and isoform-specific biological functions[5][8][9].
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Subcellular Localization
Cell membrane,Nucleus,Lysosome,Cytoplasm,Photoreceptor inner segment,Cell projection, cilium, photoreceptor outer segment
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Expression
Tissue_Specificity: Expressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine -
Isoforms & Post-Translational Modification
P48426 has two isomers: P48426-1: 46225 Da (predicted); P48426-2: 39802 Da (predicted).
Phosphorylated in tyrosines -
Subunit
Homodimer (PubMed:32130941)
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SwissProt ID
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Synonyms
PI5P4KA; PIP5K2A; PIP5KII-alpha; PIP5KIIA; PIPK
Documentation
[1]. Kiros M, et al. Trends in HIV-1 pretreatment drug resistance and HIV-1 variant dynamics among antiretroviral therapy-naive Ethiopians from 2003 to 2018: a pooled sequence analysis. Virol J. 2023 Oct 25;20(1):243. [Content Brief]
[2]. Huang Y, et al. The adjuvant treatment role of ω-3 fatty acids by regulating gut microbiota positively in the acne vulgaris. J Dermatolog Treat. 2024 Dec;35(1):2299107. [Content Brief]
[3]. Uniprotkb.
[4]. Burger ML, et al. Antigen dominance hierarchies shape TCF1+ progenitor CD8 T cell phenotypes in tumors. Cell. 2021 Sep 16;184(19):4996-5014.e26. [Content Brief]
[5]. Isaksen TJ, et al. Repulsive Guidance Molecule A Suppresses Adult Neurogenesis. Stem Cell Reports. 2020 Apr 14;14(4):677-691. [Content Brief]
[6]. Kotschy A, et al. The MCL1 inhibitor S63845 is tolerable and effective in diverse cancer models. Nature. 2016 Oct 27;538(7626):477-482. [Content Brief]
[7]. Mathre S, et al. Functional analysis of the biochemical activity of mammalian phosphatidylinositol 5 phosphate 4-kinase enzymes. Biosci Rep. 2019 Feb 19;39(2):BSR20182210. [Content Brief]
[8]. Wortmann L, et al. Discovery and Characterization of the Potent and Highly Selective 1,7-Naphthyridine-Based Inhibitors BAY-091 and BAY-297 of the Kinase PIP4K2A. J Med Chem. 2021 Nov 11;64(21):15883-15911. [Content Brief]
[9]. Li YT, et al. Primary cytoreductive surgery or interval cytoreductive surgery. Taiwan J Obstet Gynecol. 2024 Nov;63(6):811-813. [Content Brief]
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