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TIP60 Antibody (YA4411)

(Synonyms: TIP; ESA1; PLIP; KAT5; cPLA2; HTATIP; ZC2HC5; HTATIP1)
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Based on 1 Customer Validation

TIP60 Antibody (YA4411) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TIP60.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TIP60 Antibody (YA4411)
  • TIP60 Antibody (YA4411) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

TIP60 Antibody (YA4411) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TIP60.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 59 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 59 kDa
Immunogen

Purified recombinant fragment of human TIP60 (AA: 18-208) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TIP60 Antibody (YA4411)
    Western blot analysis was performed on protein extracts (30 μg) from HepG2 (lane 2) and Ramos (lane 3) using TIP60 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Histone acetyltransferase TIP60 (KAT5) is a member of the MYST family of lysine acetyltransferases and functions as the catalytic core of the evolutionarily conserved NuA4 chromatin-modifying complex, where it promotes histone acetylation and transcriptional regulation[1][2]. Mechanistically, TIP60 plays a central role in the DNA damage response by interacting with and acetylating key DNA repair regulators, including ataxia-telangiectasia mutated (ATM), thereby facilitating ATM activation following DNA double-strand breaks[1][3][4]. Following DNA damage, the NuA4/TIP60 complex is recruited to chromatin surrounding DNA lesions and contributes to chromatin remodeling events that support efficient DNA repair[1][5]. TIP60 also regulates the acetylation of multiple non-histone proteins involved in genome maintenance, highlighting its broad role in preserving cell viability and genomic stability[2]. In disease contexts, reduced TIP60 activity or expression has been associated with genomic instability, and experimental evidence supports a tumor-suppressive role for TIP60 in breast cancer models[6]. Beyond cancer, genetic studies demonstrate that TIP60 is required for neural stem cell maintenance, neurogenesis, and normal brain development, indicating functions that extend beyond canonical DNA repair pathways[7]. Compared with related MYST acetyltransferases, TIP60 is distinguished by its direct coupling of chromatin sensing and ATM-dependent DNA damage signaling, making it a widely used experimental model for investigating chromatin-regulated genome surveillance mechanisms[1][3][4].

  • Subcellular Localization

    Nucleus; Chromosome; Cytoplasm; Chromosome, centromere, kinetochore; Cytoplasm, cytoskeleton, spindle pole; Nucleus, nucleolus; Cytoplasm, perinuclear region; Cytoplasm

  • Isoforms & Post-Translational Modification

    Q92993 has 4 isomers: Q92993-1: 58582 Da (predicted); Q92993-2: 53077 Da (predicted); Q92993-3: 61798 Da (predicted); Q92993-4: 56292 Da (predicted).
    Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase (PubMed:12468530, PubMed:26829474, PubMed:29335245). The phosphorylated form has a higher activity (PubMed:12468530, PubMed:29335245). Phosphorylation at Ser-90 by CDK1 or CDK9 is a prerequisite for phosphorylation at Ser-86 by GSK3 (PubMed:26829474, PubMed:30704899). Phosphorylation at Ser-86 by GSK3 (GSK3A or GSK3B) activates acetyltransferase and acyltransferase activities (PubMed:30704899). Phosphorylation at Ser-90 by CDK9 promotes KAT5 recruitment to chromatin (PubMed:29335245). Phosphorylation by VRK1 following DNA damage promotes KAT5 association with chromatin and histone acetyltransferase activity (PubMed:33076429);Autoacetylated (PubMed:20100829, PubMed:24835996, PubMed:25301942, PubMed:26291311, PubMed:33938178). Autoacetylation is required for histone acetyltransferase activity (PubMed:20100829, PubMed:25865756). Autoacetylation at Lys-327 is facilitated by interaction with EP300/p300: it prevents ubiquitination and subsequent degradation by the proteasome and promotes acetylation of target proteins (PubMed:24835996). Deacetylated by HDAC3 and SIRT1 (PubMed:20100829, PubMed:25301942). Deacetylation by HDAC3 promotes its ubiquitination and cytoplasmic localization (PubMed:25301942);Sumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies (PubMed:17704809). Sumoylation with SUMO2 by PIAS4 at Lys-430 promotes repair of DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed:32832608). Sumoylation by PIAS4 impairs interaction with PRKDC, inhibiting non-homologous end joining (NHEJ)-mediated repair of DSBs, thereby facilitating HR (PubMed:32832608). Desumoylated by SENP3 (PubMed:32832608);Ubiquitinated by MDM2, leading to its proteasome-dependent degradation (PubMed:11927554, PubMed:24835996). Ubiquitination is prevented by autoacetylation at Lys-327 (PubMed:24835996). Ubiquitinated following deacetylation by HDAC3, leading to cytoplasmic localization (PubMed:25301942). Deubiquitinated by USP7 following interaction with ATF3, promoting its stabilization (PubMed:25865756);(Microbial infection) In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation

  • Subunit

    Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6.

  • SwissProt ID

    Q92993

  • Gene ID
    10524 [NCBI]
  • Synonyms

    TIP; ESA1; PLIP; KAT5; cPLA2; HTATIP; ZC2HC5; HTATIP1

[1]. Squatrito M, et al. Tip60 in DNA damage response and growth control: many tricks in one HAT. Trends Cell Biol. 2006 Sep;16(9):433-42. [Content Brief]

[2]. Sun Y, et al. Tip60: connecting chromatin to DNA damage signaling. Cell Cycle. 2010 Mar 1;9(5):930-6. [Content Brief]

[3]. KAT5 phosphorylation links chromatin to ATM activation. Cancer Discov. 2013 Jul;3(7):OF18. [Content Brief]

[4]. TIP60 gene information from NCBI.

[5]. Liu J, et al. Metabolic modulation of chromatin: implications for DNA repair and genomic integrity. Front Genet. 2013 Sep 17;4:182. [Content Brief]

[6]. Bassi C, et al. The acetyltransferase Tip60 contributes to mammary tumorigenesis by modulating DNA repair. Cell Death Differ. 2016 Jul;23(7):1198-208. [Content Brief]

[7]. Tominaga K, et al. Tip60/KAT5 Histone Acetyltransferase Is Required for Maintenance and Neurogenesis of Embryonic Neural Stem Cells. Int J Mol Sci. 2023 Jan 20;24(3):2113. [Content Brief]

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TIP60 Antibody (YA4411) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from HepG2 (lane 2) and Ramos (lane 3) using TIP60 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • TIP60 Antibody (YA4411) Product Image
    1/16
  • Experimental Validation Results for TIP60 Antibody (YA4411)
  • TIP60 Antibody (YA4411) Product Image
  • Experimental Validation Results for TIP60 Antibody (YA4411)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from HepG2 (lane 2) and Ramos (lane 3) using TIP60 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for TIP60 Antibody (YA4411)
  • TIP60 Antibody (YA4411) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from HepG2 (lane 2) and Ramos (lane 3) using TIP60 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • TIP60 Antibody (YA4411) Product Image WB