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RRM1 Antibody (YA1883)

(Synonyms: RRM1; R1; RIR1; RR1)
Customer Review

Based on 1 Customer Validation

RRM1 Antibody (YA1883) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RRM1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RRM1 Antibody (YA1883)
  • RRM1 Antibody (YA1883) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200 1:20

Product Details

Description

RRM1 Antibody (YA1883) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RRM1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 90 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 90 kDa
Species Reactivity Database

Entrez Gene: 6240 Human

SwissProt: P23921 Human

Immunogen

A synthetic peptide of human RRM1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104252

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RRM1 Antibody (YA1883)
    Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), A549 (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), and Hela (lane 4, 15 μg) using RRM1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    RRM1 is a Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides

  • Subcellular Localization

    Cytoplasm

  • Subunit

    Heterodimer of a large and a small subunit. Heterodimer with small subunit RRM2 or RRM2B. The heterodimer with RRM2 has higher catalytic activity than the heterodimer with RRM2B. Interacts with AHCYL1 which inhibits its activity

  • SwissProt ID

    P23921

  • Gene ID
    6240 [NCBI]
  • Synonyms

    RRM1; R1; RIR1; RR1

  • Research Field

    Epigenetics and Nuclear Signaling

RRM1 Antibody (YA1883) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), A549 (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), and Hela (lane 4, 15 μg) using RRM1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • RRM1 Antibody (YA1883) Product Image
    1/16
  • Experimental Validation Results for RRM1 Antibody (YA1883)
  • RRM1 Antibody (YA1883) Product Image
  • Experimental Validation Results for RRM1 Antibody (YA1883)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), A549 (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), and Hela (lane 4, 15 μg) using RRM1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for RRM1 Antibody (YA1883)
  • RRM1 Antibody (YA1883) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), A549 (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), and Hela (lane 4, 15 μg) using RRM1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • RRM1 Antibody (YA1883) Product Image WB