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SEC61A Antibody (YA6762)

(Synonyms: chunp6898 antibody; fb62c11 antibody; HSEC61 antibody; protein transport protein SEC61 alpha subunit antibody; protein transport protein Sec61 subunit alpha antibody; Protein transport protein Sec61 subunit alpha isoform 1 antibody; S61A1_HUMAN antibody; SEC 61 antibody; SEC 61A antibody; Sec61 alpha 1 antibody; chunp6898 antibody; fb62c11 antibody; HSEC61 antibody; protein transport protein SEC61 alpha subunit antibody; protein transport protein Sec61 subunit alpha antibody; Protein transport protein Sec61 subunit alpha isoform 1 antibody; S61A1_HUMAN antibody; SEC 61 antibody; SEC 61A antibody; Sec61 alpha 1 antibody; Sec61 alpha 1 subunit antibody; Sec61 alpha 1 subunit (S. cerevisiae) antibody; Sec61 alpha-1 antibody; SEC61 antibody; Sec61 homolog antibody; SEC61A1 antibody; sec61aa antibody; )
Customer Review

Based on 1 Customer Validation

SEC61A Antibody (YA6762) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SEC61A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SEC61A Antibody (YA6762)
  • SEC61A Antibody (YA6762) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000 1:200

Product Details

Description

SEC61A Antibody (YA6762) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SEC61A.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52 kDa
Immunogen

Recombinant protein within Human SEC61A2 aa 327-476 / 476.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SEC61A Antibody (YA6762)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A431 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using SEC61A Antibody (HY-P87069) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    SEC61A component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides. May cooperate with auxiliary protein SEC62, SEC63 and HSPA5/BiP to enable post-translational transport of small presecretory proteins. The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins: it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex. The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER. Controls the passive efflux of calcium ions from the ER lumen to the cytosol through SEC61 channel, contributing to the maintenance of cellular calcium homeostasis. Plays a critical role in nephrogenesis, specifically at pronephros stage[1][2][3][4][5][6][7][8].

  • Subcellular Localization

    Endoplasmic reticulum membrane

  • Expression


    Tissue_Specificity: Expressed in proximal and distal tubules in kidney (at protein level)

  • Isoforms & Post-Translational Modification

    P61619 has two isomers: P61619-1: 52265 Da (predicted); P61619-3: 38849 Da (predicted).

  • Subunit

    The SEC61 channel-forming translocon complex consists of channel-forming core components SEC61A1, SEC61B and SEC61G and different auxiliary components such as SEC62 and SEC63 (PubMed:36697828)

  • SwissProt ID

    P61619

  • Gene ID
    29927 [NCBI]
  • Synonyms

    chunp6898 antibody; fb62c11 antibody; HSEC61 antibody; protein transport protein SEC61 alpha subunit antibody; protein transport protein Sec61 subunit alpha antibody; Protein transport protein Sec61 subunit alpha isoform 1 antibody; S61A1_HUMAN antibody; SEC 61 antibody; SEC 61A antibody; Sec61 alpha 1 antibody; chunp6898 antibody; fb62c11 antibody; HSEC61 antibody; protein transport protein SEC61 alpha subunit antibody; protein transport protein Sec61 subunit alpha antibody; Protein transport protein Sec61 subunit alpha isoform 1 antibody; S61A1_HUMAN antibody; SEC 61 antibody; SEC 61A antibody; Sec61 alpha 1 antibody; Sec61 alpha 1 subunit antibody; Sec61 alpha 1 subunit (S. cerevisiae) antibody; Sec61 alpha-1 antibody; SEC61 antibody; Sec61 homolog antibody; SEC61A1 antibody; sec61aa antibody;

SEC61A Antibody (YA6762) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A431 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using SEC61A Antibody (HY-P87069) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • SEC61A Antibody (YA6762) Product Image
    1/16
  • Experimental Validation Results for SEC61A Antibody (YA6762)
  • SEC61A Antibody (YA6762) Product Image
  • Experimental Validation Results for SEC61A Antibody (YA6762)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A431 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using SEC61A Antibody (HY-P87069) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SEC61A Antibody (YA6762)
  • SEC61A Antibody (YA6762) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A431 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using SEC61A Antibody (HY-P87069) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • SEC61A Antibody (YA6762) Product Image WB