SEC61A Antibody (YA6762)

Cat. No.: HY-P87069: Data Sheet COA
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SEC61A Antibody (YA6762) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SEC61A.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

SEC61A Antibody (YA6762) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SEC61A.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 52 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P61619

Gene ID

29927 [NCBI]

Immunogen

Recombinant protein within Human SEC61A2 aa 327-476 / 476.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A431 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using SEC61A Antibody (HY-P87069) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER) (PubMed:12475939, PubMed:22375059, PubMed:28782633, PubMed:29719251, PubMed:32814900). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides (PubMed:22375059, PubMed:28782633, PubMed:29719251). May cooperate with auxiliary protein SEC62, SEC63 and HSPA5/BiP to enable post-translational transport of small presecretory proteins (PubMed:22375059, PubMed:29719251). The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins: it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex (PubMed:32820719, PubMed:36261522). The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER (PubMed:8616892). Controls the passive efflux of calcium ions from the ER lumen to the cytosol through SEC61 channel, contributing to the maintenance of cellular calcium homeostasis (PubMed:28782633). Plays a critical role in nephrogenesis, specifically at pronephros stage (By similarity)

Subcellular Localization：Endoplasmic reticulum membrane

Expression：
Tissue_Specificity: Expressed in proximal and distal tubules in kidney (at protein level)

Isoforms & Post-Translational Modification：P61619 has two isomers: P61619-1: 52265 Da (predicted); P61619-3: 38849 Da (predicted).

Subunit：The SEC61 channel-forming translocon complex consists of channel-forming core components SEC61A1, SEC61B and SEC61G and different auxiliary components such as SEC62 and SEC63 (PubMed:36697828)

Synonyms

chunp6898 antibody; fb62c11 antibody; HSEC61 antibody; protein transport protein SEC61 alpha subunit antibody; protein transport protein Sec61 subunit alpha antibody; Protein transport protein Sec61 subunit alpha isoform 1 antibody; S61A1_HUMAN antibody; SEC 61 antibody; SEC 61A antibody; Sec61 alpha 1 antibody; chunp6898 antibody; fb62c11 antibody; HSEC61 antibody; protein transport protein SEC61 alpha subunit antibody; protein transport protein Sec61 subunit alpha antibody; Protein transport protein Sec61 subunit alpha isoform 1 antibody; S61A1_HUMAN antibody; SEC 61 antibody; SEC 61A antibody; Sec61 alpha 1 antibody; Sec61 alpha 1 subunit antibody; Sec61 alpha 1 subunit (S. cerevisiae) antibody; Sec61 alpha-1 antibody; SEC61 antibody; Sec61 homolog antibody; SEC61A1 antibody; sec61aa antibody;

Documentation

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Data Sheet (231 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)