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delta Tubulin Antibody

(Synonyms: TUBD1,TUBD,FLJ12709,TBD_HUMAN antibody,Tubulin delta chain antibod)
Customer Review

Based on 1 Customer Validation

delta Tubulin Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to delta Tubulin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in pH 7.4 PBS, 0.05% NaN3, 40% Glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for delta Tubulin Antibody
  • Experimental Validation Results for delta Tubulin Antibody
  • Experimental Validation Results for delta Tubulin Antibody
  • delta Tubulin Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

delta Tubulin Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to delta Tubulin.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 51 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 51 kDa
Species Reactivity Database
Immunogen

Fusion protein of human TUBD1.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3676577

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in pH 7.4 PBS, 0.05% NaN3, 40% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for delta Tubulin Antibody
    Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using delta Tubulin Antibody(HY-P83669) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for delta Tubulin Antibody
    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using delta Tubulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83669, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for delta Tubulin Antibody
    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using delta Tubulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83669, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    delta Tubulin acts as a positive regulator of hedgehog signaling and regulates ciliary function

  • Subcellular Localization

    Nucleus; Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole; Cell projection, cilium

  • Isoforms & Post-Translational Modification

    Q9UJT1 has 6 isomers: Q9UJT1-1: 51034 Da (predicted); Q9UJT1-2: 44658 Da (predicted); Q9UJT1-3: 22381 Da (predicted); Q9UJT1-4: 27074 Da (predicted); Q9UJT1-5: 44555 Da (predicted); Q9UJT1-6: 39525 Da (predicted).

  • Subunit

    Found in a complex with TEDC1, TEDC2, TUBE1 and TUBD1

  • SwissProt ID

    Q9UJT1

  • Gene ID
    51174 [NCBI]
  • Synonyms

    TUBD1,TUBD,FLJ12709,TBD_HUMAN antibody,Tubulin delta chain antibod

  • Research Field

    Signal Transduction

delta Tubulin Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using delta Tubulin Antibody(HY-P83669) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using delta Tubulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83669, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using delta Tubulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83669, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • delta Tubulin Antibody Product Image
    1/16
  • Experimental Validation Results for delta Tubulin Antibody
  • Experimental Validation Results for delta Tubulin Antibody
  • Experimental Validation Results for delta Tubulin Antibody
  • delta Tubulin Antibody Product Image
  • Experimental Validation Results for delta Tubulin Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using delta Tubulin Antibody(HY-P83669) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for delta Tubulin Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using delta Tubulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83669, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for delta Tubulin Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using delta Tubulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83669, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for delta Tubulin Antibody
  • Experimental Validation Results for delta Tubulin Antibody
  • Experimental Validation Results for delta Tubulin Antibody
  • delta Tubulin Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using delta Tubulin Antibody(HY-P83669) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • delta Tubulin Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using delta Tubulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83669, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • delta Tubulin Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using delta Tubulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83669, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • delta Tubulin Antibody Product Image WB
  • delta Tubulin Antibody Product Image IHC-P
  • delta Tubulin Antibody Product Image IHC-P