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TAGLN/Transgelin Antibody (YA6128)

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Based on 1 Customer Validation

TAGLN/Transgelin Antibody (YA6128) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TAGLN/Transgelin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG/Kappa

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TAGLN/Transgelin Antibody (YA6128)
  • TAGLN/Transgelin Antibody (YA6128) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000

Product Details

Description

TAGLN/Transgelin Antibody (YA6128) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TAGLN/Transgelin.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 23 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG/Kappa

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TAGLN/Transgelin Antibody (YA6128)
    Western blot analysis of extracts from Human colon cancer (lane 1(20μg)) 、Human cervical cancer (lane 2(20μg)) 、Mouse colon (lane 3(20μg)) and Rat colon (lane 4(20μg)) TAGLN Antibody (HY-P86436) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    TAGLN/Transgelin is an Actin cross-linking/gelling protein. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence

  • Subcellular Localization

    Cytoplasm

  • Expression

    Induction: Overexpressed in senescent human fibroblasts

  • Isoforms & Post-Translational Modification

    Q01995: 201 amino acids, molecular weight 22611 Da.
    Phosphorylated at Ser-32 and Ser-36 by IKKA/CHUK and IKKB/IKBKB; disables inhibition of NF-kappa-B DNA-binding activity (PubMed:10329681, PubMed:10882136, PubMed:7628694, PubMed:7796813, PubMed:7878466, PubMed:8631829, PubMed:9701247)丨Polyubiquitinated at Lys-21 and/or Lys-22 following phosphorylation at Ser-32 and Ser-36 (PubMed:10329681, PubMed:20347421, PubMed:7479976, PubMed:8631829, PubMed:9701247)丨Sumoylated; sumoylation requires the presence of the nuclear import signal丨Hydroxylated by HIF1AN丨(Microbial infection) Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation

  • SwissProt ID

    Q01995

  • Gene ID
    6876 [NCBI]

TAGLN/Transgelin Antibody (YA6128) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Human colon cancer (lane 1(20μg)) 、Human cervical cancer (lane 2(20μg)) 、Mouse colon (lane 3(20μg)) and Rat colon (lane 4(20μg)) TAGLN Antibody (HY-P86436) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • TAGLN/Transgelin Antibody (YA6128) Product Image
    1/16
  • Experimental Validation Results for TAGLN/Transgelin Antibody (YA6128)
  • TAGLN/Transgelin Antibody (YA6128) Product Image
  • Experimental Validation Results for TAGLN/Transgelin Antibody (YA6128)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Human colon cancer (lane 1(20μg)) 、Human cervical cancer (lane 2(20μg)) 、Mouse colon (lane 3(20μg)) and Rat colon (lane 4(20μg)) TAGLN Antibody (HY-P86436) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TAGLN/Transgelin Antibody (YA6128)
  • TAGLN/Transgelin Antibody (YA6128) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Human colon cancer (lane 1(20μg)) 、Human cervical cancer (lane 2(20μg)) 、Mouse colon (lane 3(20μg)) and Rat colon (lane 4(20μg)) TAGLN Antibody (HY-P86436) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • TAGLN/Transgelin Antibody (YA6128) Product Image WB