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PDE1B Antibody (YA1553)

(Synonyms: PDE1B1; PDES1B; HEL-S-79p)
Customer Review

Based on 1 Customer Validation

PDE1B Antibody (YA1553) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDE1B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PDE1B Antibody (YA1553)
  • PDE1B Antibody (YA1553) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

PDE1B Antibody (YA1553) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDE1B.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 61 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 61 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human PDE1B

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103909

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PDE1B Antibody (YA1553)
    Western blot analysis of extracts from PC-12 (lane 2(20μg), PC-12 (lane 3(40μg), using PDE1B Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PDE1B is a Cyclic nucleotide phosphodiesterase with a dual specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate[1][2][3].

  • Subcellular Localization

    Cytoplasm, cytosol

  • Isoforms & Post-Translational Modification

    Q01064 has 2 isomers: Q01064-1: 61380 Da (predicted); Q01064-2: 59051 Da (predicted).

  • Subunit

    Homodimer

  • SwissProt ID

    Q01064

  • Gene ID
    5153 [NCBI]
  • Synonyms

    PDE1B1; PDES1B; HEL-S-79p

  • Research Field

    Signal Transduction

PDE1B Antibody (YA1553) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from PC-12 (lane 2(20μg), PC-12 (lane 3(40μg), using PDE1B Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PDE1B Antibody (YA1553) Product Image
    1/16
  • Experimental Validation Results for PDE1B Antibody (YA1553)
  • PDE1B Antibody (YA1553) Product Image
  • Experimental Validation Results for PDE1B Antibody (YA1553)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from PC-12 (lane 2(20μg), PC-12 (lane 3(40μg), using PDE1B Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PDE1B Antibody (YA1553)
  • PDE1B Antibody (YA1553) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from PC-12 (lane 2(20μg), PC-12 (lane 3(40μg), using PDE1B Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PDE1B Antibody (YA1553) Product Image WB