MLK3 Antibody (YA3237)
(Synonyms: MAP3K11; MLK3; PTK1; SPRK; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase)
Based on 1 Customer Validation
MLK3 Antibody (YA3237) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MLK3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 |
Product Details
MLK3 Antibody (YA3237) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MLK3.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 93 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 93 kDa
A synthetic peptide of human MLK3
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MLK3 activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle
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Subcellular Localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
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Expression
Tissue_specificity:Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and human lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain -
Isoforms & Post-Translational Modification
Q16584 has 2 isomers: Q16584-1: 92688 Da (predicted); Q16584-2: 65344 Da (predicted).
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42 -
Subunit
Homodimer; undergoes dimerization during activation (PubMed:9829970). Interacts with MAP2K7/MKK7 (PubMed:9003778).
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SwissProt ID
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Synonyms
MAP3K11; MLK3; PTK1; SPRK; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase
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Research Field
Signal Transduction
Documentation
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