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Cat. No.: HY-P87750

Smad2 Antibody (YA7435)

Smad2 Antibody (YA7435) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Smad2.

For research use only. We do not sell to patients.

Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Price

Stock

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	WB WB: Western Blot	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence	ELISA ELISA: Enzyme Linked Immunosorbent Assay	IP IP: Immunoprecipitation
Dilution Ratio	1:1000-1:5000	1:200-1:1000	1:5000-1:20000	1:50-1:200,

Description

Smad2 Antibody (YA7435) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Smad2.

Host Rabbit
Clonality Monoclonal,Recombinant
Species Reactivity
Human, Mouse, Rat
Observed Molecular Weight

Observed band size: 58 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 52 kDa

Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG, Kappa

Appearance

Liquid
Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Function

Smad2 is a Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma^[1].
Subcellular Localization

Cytoplasm,Nucleus
Expression

Tissue_Specificity: Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta
Isoforms & Post-Translational Modification

Q15796 has two isomers: Q15796-1: 52306 Da (predicted); Q15796-2: 48956 Da (predicted).
Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255丨In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation丨Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity
Subunit

Monomer; in the absence of TGF-beta (PubMed:9670020)
SwissProt ID

Q15796