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TUBB2A Antibody (YA4256)

(Synonyms: TUBB; TUBB2; dJ40E16.7 ; Tubulin beta-2A chain)
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Based on 1 Customer Validation

TUBB2A Antibody (YA4256) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TUBB2A.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for TUBB2A Antibody (YA4256)
  • Experimental Validation Results for TUBB2A Antibody (YA4256)
  • Experimental Validation Results for TUBB2A Antibody (YA4256)
  • TUBB2A Antibody (YA4256) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

TUBB2A Antibody (YA4256) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TUBB2A.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 50 kDa
Immunogen

Purified recombinant fragment of human TUBB2A (AA: 25-187) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718936

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TUBB2A Antibody (YA4256)
    Western blot analysis of extracts from 293T(lane 2(20μg) ,SH-SY5Y(lane 3(20μg) ,Jurkat(lane 4(20μg)and A549(lane 5(20μg) using TUBB2A Antibody（HY-P84559）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TUBB2A Antibody (YA4256)
    Western blot analysis of extracts from K562(lane 1(40μg) )
  • Experimental Validation Results for TUBB2A Antibody (YA4256)
    Flow cytometric analysis of 1X10^6 Hela cells labeling TUBB2A Antibody(HY-P84559

Background

  • Function

    TUBB2A is a Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin

  • Subcellular Localization

    Cytoplasm, cytoskeleton

  • Expression


    Tissue_specificity:It is highly expressed in the brain, accounting for 30% of all β-tubulins.

  • Subunit

    Interacts with ZNRF1 (By similarity).

  • SwissProt ID

    Q13885

  • Gene ID
    7280 [NCBI]
  • Synonyms

    TUBB; TUBB2; dJ40E16.7 ; Tubulin beta-2A chain

TUBB2A Antibody (YA4256) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from 293T(lane 2(20μg) ,SH-SY5Y(lane 3(20μg) ,Jurkat(lane 4(20μg)and A549(lane 5(20μg) using TUBB2A Antibody（HY-P84559）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 1(40μg) )

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling TUBB2A Antibody(HY-P84559

  • TUBB2A Antibody (YA4256) Product Image
    1/16
  • Experimental Validation Results for TUBB2A Antibody (YA4256)
  • Experimental Validation Results for TUBB2A Antibody (YA4256)
  • Experimental Validation Results for TUBB2A Antibody (YA4256)
  • TUBB2A Antibody (YA4256) Product Image
  • Experimental Validation Results for TUBB2A Antibody (YA4256)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from 293T(lane 2(20μg) ,SH-SY5Y(lane 3(20μg) ,Jurkat(lane 4(20μg)and A549(lane 5(20μg) using TUBB2A Antibody（HY-P84559）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TUBB2A Antibody (YA4256)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 1(40μg) )
  • Experimental Validation Results for TUBB2A Antibody (YA4256)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling TUBB2A Antibody(HY-P84559
  • Experimental Validation Results for TUBB2A Antibody (YA4256)
  • Experimental Validation Results for TUBB2A Antibody (YA4256)
  • Experimental Validation Results for TUBB2A Antibody (YA4256)
  • TUBB2A Antibody (YA4256) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from 293T(lane 2(20μg) ,SH-SY5Y(lane 3(20μg) ,Jurkat(lane 4(20μg)and A549(lane 5(20μg) using TUBB2A Antibody（HY-P84559）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • TUBB2A Antibody (YA4256) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 1(40μg) )

  • TUBB2A Antibody (YA4256) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling TUBB2A Antibody(HY-P84559

  • TUBB2A Antibody (YA4256) Product Image WB
  • TUBB2A Antibody (YA4256) Product Image WB
  • TUBB2A Antibody (YA4256) Product Image FC