Fyn Antibody (YA4868)
(Synonyms: FYN; Tyrosine-protein kinase Fyn; Proto-oncogene Syn; Proto-oncogene c-Fyn; Src-like kinase; SLK; p59-Fyn)Based on 1 Customer Validation
Fyn Antibody (YA4868) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Fyn.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P, ICC/IF, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
Fyn Antibody (YA4868) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Fyn.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 61 kDa;
Purified recombinant fragment of human Fyn expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Fyn is a Src family tyrosine kinase represented in brain by the neuronal isoform p59FynB, where Src and Fyn constitute the two main tyrosine kinases involved in neuronal growth and myelination[1]. Mechanistically, Fyn participates in Src-family signaling modules that regulate inflammation, apoptosis, neuroinflammation, and epileptogenesis in experimental systems[2][3]. In pulmonary endothelial inflammation models, fyn knockdown increased caspase-3 activity, reduced viable cell numbers, and intensified LPS-induced lung injury in fyn−/− mice, identifying Fyn as a negative regulator of inflammation-induced apoptosis[2]. In temporal lobe epilepsy models, the Fyn-PKCδ pathway linked status epilepticus to neuroinflammation and epileptogenesis, while saracatinib reduced seizures, gliosis, neurodegeneration, phosphorylated Fyn/Src-419, PKCδ-tyr311, nitrooxidative stress, and proinflammatory cytokines[3]. Compared with related isoforms, Fyn showed an opposite endothelial apoptosis phenotype to Yes, because yes knockdown lowered caspase-3 activity and increased viable cells under cytomix stimulation[2]. For experimental applications, saracatinib functions as a Src/Fyn tyrosine kinase inhibitor for probing epileptogenesis-associated neuroinflammatory and nitrooxidative pathways[3].
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Subcellular Localization
Cytoplasm; Nucleus; Cell membrane; Perikaryon
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Expression
Tissue_specificity:Isomer 1 is highly expressed in the brain. Isomer 2 is expressed in hematopoietic lineage cells, especially in T lymphocytes. -
Isoforms & Post-Translational Modification
P06241 has 3 isomers: P06241-1: 60762 Da (predicted); P06241-2: 60141 Da (predicted); P06241-3: 54513 Da (predicted).
Autophosphorylated at Tyr-420 (By similarity). Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state (PubMed:1699196). PTPRC/CD45 dephosphorylates Tyr-531 leading to activation (PubMed:1533589). Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus (PubMed:15537652). Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling (PubMed:22080863). Phosphorylated at tyrosine residues, which can be enhanced by NTN1 (By similarity);Palmitoylated. Palmitoylation at Cys-3 and Cys-6, probably by ZDHHC21, regulates subcellular location -
Subunit
Interacts (via its SH3 domain) with PIK3R1 and PRMT8. Interacts with FYB1, PAG1, and SH2D1A. Interacts with CD79A (tyrosine-phosphorylated form); the interaction increases FYN activity.
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SwissProt ID
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Synonyms
FYN; Tyrosine-protein kinase Fyn; Proto-oncogene Syn; Proto-oncogene c-Fyn; Src-like kinase; SLK; p59-Fyn
Documentation
[1]. Rouer E. Les isoformes neuronales des tyrosines kinases Src, et al. Les isoformes neuronales des tyrosines kinases Src, Fyn et Lck : rôle particulier de la p56lckN dans la survie neuronale [Neuronal isoforms of Src, Fyn and Lck tyrosine kinases: A specific role for p56lckN in neuron protection]. C R Biol. 2010 Jan;333(1):1-10. French. [Content Brief]
[3]. Sharma S, et al. Mechanisms of disease-modifying effect of saracatinib (AZD0530), a Src/Fyn tyrosine kinase inhibitor, in the rat kainate model of temporal lobe epilepsy. Neurobiol Dis. 2021 Aug;156:105410. [Content Brief]
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