Copy Link Print Information

Integrin beta 4 Antibody (YA3821)

(Synonyms: ITGB4; GP150)
Customer Review

Based on 1 Customer Validation

Integrin beta 4 Antibody (YA3821) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Integrin beta 4.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Integrin beta 4 Antibody (YA3821) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

Integrin beta 4 Antibody (YA3821) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Integrin beta 4.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 200-210 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 202 kDa
Immunogen

Purified recombinant fragment of human CD104 (AA: extra 29-206) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
    Immunohistochemical analysis of paraffin-embedded human placenta using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
    Immunohistochemical analysis of paraffin-embedded human head and neck cancer using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
    Immunohistochemical analysis of paraffin-embedded human colon using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
    Immunohistochemical analysis of paraffin-embedded human bladder cancer using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
    Immunohistochemical analysis of paraffin-embedded human stomach using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Integrin beta 4 is an Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling[1][2][3].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell membrane; Lipid-anchor; Cell junction, hemidesmosome

  • Expression


    Tissue_specificity:Integrin α6/β4 is primarily expressed in epithelial cells. The β4D isoform is also expressed in the colon and placenta. The β4E isoform is also expressed in the epidermis, lungs, duodenum, heart, spleen, and stomach.

  • Isoforms & Post-Translational Modification

    P16144 has 5 isomers: P16144-1: 202167 Da (predicted); P16144-2: 195013 Da (predicted); P16144-3: 200725 Da (predicted); P16144-4: 194466 Da (predicted); P16144-5: 106779 Da (predicted).
    Palmitoylated by DHHC3 at several cysteines of the membrane-proximal region, enhancing stability and cell surface expression. Palmitoylation also promotes secondary association with tertaspanins

  • Subunit

    Heterodimer of an alpha and a beta subunit. Beta-4 associates with alpha-6. Interacts (via cytoplasmic region) with COL17A1 (via cytoplasmic region). Interacts (via cytoplasmic region) with DST isoform 3 (via N-terminus). Isoform beta-4a interacts (via cytoplasmic domain) with DST (via N-terminus). Interacts with RAC1. ITGA6:ITGB4 is found in a ternary complex with NRG1 and ERBB3 (PubMed:20682778). ITGA6:ITGB4 is found in a ternary complex with IGF1 and IGF1R (PubMed:22351760). ITGA6:ITGB4 interacts with IGF2 (PubMed:28873464). Interacts with TMEM268; this interaction prevents ITGB4 degradation (PubMed:30361615)

  • SwissProt ID

    P16144

  • Gene ID
    3691 [NCBI]
  • Synonyms

    ITGB4; GP150

Integrin beta 4 Antibody (YA3821) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human head and neck cancer using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bladder cancer using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Integrin beta 4 Antibody (YA3821) Product Image
    1/16
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Integrin beta 4 Antibody (YA3821) Product Image
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human head and neck cancer using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bladder cancer using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Experimental Validation Results for Integrin beta 4 Antibody (YA3821)
  • Integrin beta 4 Antibody (YA3821) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Integrin beta 4 Antibody (YA3821) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human head and neck cancer using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Integrin beta 4 Antibody (YA3821) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Integrin beta 4 Antibody (YA3821) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Integrin beta 4 Antibody (YA3821) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human bladder cancer using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Integrin beta 4 Antibody (YA3821) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human stomach using Integrin beta 4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84124, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Integrin beta 4 Antibody (YA3821) Product Image IHC-P
  • Integrin beta 4 Antibody (YA3821) Product Image IHC-P
  • Integrin beta 4 Antibody (YA3821) Product Image IHC-P
  • Integrin beta 4 Antibody (YA3821) Product Image IHC-P
  • Integrin beta 4 Antibody (YA3821) Product Image IHC-P
  • Integrin beta 4 Antibody (YA3821) Product Image IHC-P