Integrin beta 4 Antibody (YA3821)

Cat. No.: HY-P84124: Data Sheet COA
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Integrin beta 4 Antibody (YA3821) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Integrin beta 4.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Integrin beta 4 Antibody (YA3821) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Integrin beta 4.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 202 kDa;

Observed band size: 200-210 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P16144

Gene ID

3691 [NCBI]

Immunogen

Purified recombinant fragment of human CD104 (AA: extra 29-206) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell membrane; Lipid-anchor; Cell junction, hemidesmosome

Expression：
Tissue_specificity:Integrin α6/β4 is primarily expressed in epithelial cells. The β4D isoform is also expressed in the colon and placenta. The β4E isoform is also expressed in the epidermis, lungs, duodenum, heart, spleen, and stomach.

Isoforms & Post-Translational Modification：P16144 has 5 isomers: P16144-1: 202167 Da (predicted); P16144-2: 195013 Da (predicted); P16144-3: 200725 Da (predicted); P16144-4: 194466 Da (predicted); P16144-5: 106779 Da (predicted).
Palmitoylated by DHHC3 at several cysteines of the membrane-proximal region, enhancing stability and cell surface expression. Palmitoylation also promotes secondary association with tertaspanins

Subunit：Heterodimer of an alpha and a beta subunit. Beta-4 associates with alpha-6. Interacts (via cytoplasmic region) with COL17A1 (via cytoplasmic region). Interacts (via cytoplasmic region) with DST isoform 3 (via N-terminus). Isoform beta-4a interacts (via cytoplasmic domain) with DST (via N-terminus). Interacts with RAC1. ITGA6:ITGB4 is found in a ternary complex with NRG1 and ERBB3 (PubMed:20682778). ITGA6:ITGB4 is found in a ternary complex with IGF1 and IGF1R (PubMed:22351760). ITGA6:ITGB4 interacts with IGF2 (PubMed:28873464). Interacts with TMEM268; this interaction prevents ITGB4 degradation (PubMed:30361615)

Synonyms

ITGB4; GP150

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)