Histone H2B Antibody (YA378)

(Synonyms: H2B GL105 antibody; H2B histone family member O antibody; H2B histone family member S antibody; H2B.1 antibody; H2B.1 B antibody; H2B.b antibody; H2B.c antibody; H2B.d antibody; H2B.e antibody; H2B.f antibody; H2B.j antibody; H2B.q antibody; H2B/b antibody; H2B/c antibody; H2B/d antibody; H2B/e antibody; H2B/f antibody; H2B/j antibody; H2B/o antibody; H2B/q antibody; H2BFB antibody; H2BFC antibody; H2BFD antibody; H2BFE antibody; H2BFF antibody; H2BFJ antibody; H2BFO antibody; H2BFQ antibody; H2BFS antibody; HIRIP2 antibody; HIST1H2BB antibody; HIST1H2BD antibody; HIST1H2BH antibody; HIST1H2BL antibody; HIST1H2BM antibody; HIST1H2BN antibody; HIST2H2BE antibody; Histone H2B antibody; Histone H2B type 1 B antibody; Histone H2B type 1 D antibody; Histone H2B type 1 H antibody; Histone H2B type 1 L antibody; Histone H2B type 1 M antibody; Histone H2B type 1 N antibody; Histone H2B type 2 E antibody; histone protein antibody)
Customer Review

Based on 1 Customer Validation

Histone H2B Antibody (YA378) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Histone H2B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:2000 1:100-1:500 1:50-1:5000 1:50-1:100 Use at an assay dependent concentration.

Product Details

Description

Histone H2B Antibody (YA378) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Histone H2B.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 14 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 14 kDa
Immunogen

Synthetic peptide corresponding to Human Histone H2B.AA range:110 to the C-terminus .

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102324

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Histone H2B Antibody (YA378)
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Histone H2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80163, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Histone H2B Antibody (YA378)
    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg)using Histone H2B (HY-P80163) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80163, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Histone H2B Antibody (YA378)
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Histone H2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80163, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Histone H2B Antibody (YA378)
    Immunocytochemistry analysis of A431 cells labeling Histone H2B with Histone H2B antibody (HY-P80163) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H2B antibody (HY-P80163) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Histone H2B Antibody (YA378)
    Western blot analysis of extracts from Hela (lane 2(20μg) , Hela (lane 3(20μg),using Histone H2B Antibody (HY-P80163). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Histone H2B Antibody (YA378)
    Flow cytometric analysis of 1X10^6 Hela cells labeling Histone H2B Antibody (HY-P80163, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Histone H2B core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling; Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid

  • Subcellular Localization

    Nucleus; Chromosome

  • Subunit

    The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA

  • SwissProt ID

    O60814

  • Gene ID
  • Synonyms

    H2B GL105 antibody; H2B histone family member O antibody; H2B histone family member S antibody; H2B.1 antibody; H2B.1 B antibody; H2B.b antibody; H2B.c antibody; H2B.d antibody; H2B.e antibody; H2B.f antibody; H2B.j antibody; H2B.q antibody; H2B/b antibody; H2B/c antibody; H2B/d antibody; H2B/e antibody; H2B/f antibody; H2B/j antibody; H2B/o antibody; H2B/q antibody; H2BFB antibody; H2BFC antibody; H2BFD antibody; H2BFE antibody; H2BFF antibody; H2BFJ antibody; H2BFO antibody; H2BFQ antibody; H2BFS antibody; HIRIP2 antibody; HIST1H2BB antibody; HIST1H2BD antibody; HIST1H2BH antibody; HIST1H2BL antibody; HIST1H2BM antibody; HIST1H2BN antibody; HIST2H2BE antibody; Histone H2B antibody; Histone H2B type 1 B antibody; Histone H2B type 1 D antibody; Histone H2B type 1 H antibody; Histone H2B type 1 L antibody; Histone H2B type 1 M antibody; Histone H2B type 1 N antibody; Histone H2B type 2 E antibody; histone protein antibody

  • Research Field

    Epigenetics and Nuclear Signaling

Histone H2B Antibody (YA378) Related Classifications

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Minimum order quantity
100 mg

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