GOT2 Antibody (YA2862)

Cat. No.: HY-P83117: Data Sheet COA
SDS
User Guide for Antibodies
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GOT2 Antibody (YA2862) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GOT2.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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GOT2 Antibody (YA2862) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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GOT2 Antibody (YA2862) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GOT2.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 48 kDa;

Observed band size: 43 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P00505

Gene ID

2806 [NCBI]

Immunogen

A synthesized peptide derived from human GOT2 aa17-72.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:50

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), Rat brain (lane 4, 15 μg), Mouse liver (lane 5, 15 μg), and Rat liver (lane 6, 15 μg) using GOT2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids

Subcellular Localization：Mitochondrion matrix; Cell membrane

Expression：
Induction:Up-regulated by long-time exposure to alcohol

Isoforms & Post-Translational Modification：P00505 has 2 isomers: P00505-1: 47518 Da (predicted); P00505-2: 43030 Da (predicted).

Subunit：Homodimer

RRID

AB_3105232

Database

Entrez Gene: 2806 Human ; 14719 Mouse ; 25721 Rat

SwissProt: P00505 Human ; P05202 Mouse ; P00507 Rat

OMIM: 618721 Human

Research Field

Signal Transduction

Synonyms

KAT4; KATIV; KYAT4; mitAAT

각종 서류

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

GOT2 Antibody (YA2862) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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GOT2 Antibody (YA2862)

GOT2 Antibody (YA2862) Featured Recommendations:

Product Detail

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GOT2 Antibody (YA2862) Related Classifications

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