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GOT2 Antibody (YA2862)

(Synonyms: KAT4; KATIV; KYAT4; mitAAT)
Customer Review

Based on 1 Customer Validation

GOT2 Antibody (YA2862) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GOT2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GOT2 Antibody (YA2862)
  • GOT2 Antibody (YA2862) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50

Product Details

Description

GOT2 Antibody (YA2862) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GOT2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 48 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human GOT2 aa17-72.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105232

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GOT2 Antibody (YA2862)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), Rat brain (lane 4, 15 μg), Mouse liver (lane 5, 15 μg), and Rat liver (lane 6, 15 μg) using GOT2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    GOT2 catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids

  • Subcellular Localization

    Mitochondrion matrix; Cell membrane

  • Expression


    Induction:Up-regulated by long-time exposure to alcohol

  • Isoforms & Post-Translational Modification

    P00505 has 2 isomers: P00505-1: 47518 Da (predicted); P00505-2: 43030 Da (predicted).

  • Subunit

    Homodimer

  • SwissProt ID

    P00505

  • Gene ID
    2806 [NCBI]
  • Synonyms

    KAT4; KATIV; KYAT4; mitAAT

  • Research Field

    Signal Transduction

GOT2 Antibody (YA2862) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), Rat brain (lane 4, 15 μg), Mouse liver (lane 5, 15 μg), and Rat liver (lane 6, 15 μg) using GOT2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • GOT2 Antibody (YA2862) Product Image
    1/16
  • Experimental Validation Results for GOT2 Antibody (YA2862)
  • GOT2 Antibody (YA2862) Product Image
  • Experimental Validation Results for GOT2 Antibody (YA2862)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), Rat brain (lane 4, 15 μg), Mouse liver (lane 5, 15 μg), and Rat liver (lane 6, 15 μg) using GOT2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for GOT2 Antibody (YA2862)
  • GOT2 Antibody (YA2862) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), Rat brain (lane 4, 15 μg), Mouse liver (lane 5, 15 μg), and Rat liver (lane 6, 15 μg) using GOT2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • GOT2 Antibody (YA2862) Product Image WB