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Myelin Basic Protein Antibody (YA3271)

(Synonyms: Myelin membrane encephalitogenic protein; Myelin A1 protein)
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Myelin Basic Protein Antibody (YA3271) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myelin Basic Protein.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Myelin Basic Protein Antibody (YA3271) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Myelin Basic Protein Antibody (YA3271) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myelin Basic Protein.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 18 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human Myelin Basic Protein

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105636

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    Myelin Basic Protein is a The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation

  • Subcellular Localization

    Myelin membrane; Peripheral membrane protein; Cytoplasmic side; Nucleus

  • Expression


    Tissue_specificity:MBP isoforms are found in the central and peripheral nervous systems, while the Golli-MBP isoform is expressed in the fetal thymus, spleen, spinal cord, and cell lines derived from the immune system.

  • Isoforms & Post-Translational Modification

    P02686 has 6 isomers: P02686-1: 33117 Da (predicted); P02686-2: 21522 Da (predicted); P02686-3: 21493 Da (predicted); P02686-4: 20246 Da (predicted); P02686-5: 18591 Da (predicted); P02686-6: 17343 Da (predicted).
    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic;The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6);Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated;Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells;Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1

  • Subunit

    Homodimer. Isoform 3 exists as a homodimer

  • SwissProt ID

    P02686

  • Gene ID
    4155 [NCBI]
  • Synonyms

    Myelin membrane encephalitogenic protein; Myelin A1 protein

  • Research Field

    Neuroscience

Myelin Basic Protein Antibody (YA3271) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    1/16
  • Myelin Basic Protein Antibody (YA3271) Product Image