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PGK1 Antibody (YA862)

(Synonyms: PGKA; MIG10; HEL-S-68p)
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Based on 1 Customer Validation

PGK1 Antibody (YA862) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PGK1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in phosphate buffered saline, 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for PGK1 Antibody (YA862)
  • Experimental Validation Results for PGK1 Antibody (YA862)
  • Experimental Validation Results for PGK1 Antibody (YA862)
  • PGK1 Antibody (YA862) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

PGK1 Antibody (YA862) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PGK1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human PGK1

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103029

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in phosphate buffered saline, 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PGK1 Antibody (YA862)
    Western blot analysis of extracts from HT-29(lane 2(20ug) , NIH/3T3(lane 3(20ug) and C2C12(lane 4(20ug) using PGK1 Antibody (HY-P80784A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PGK1 Antibody (YA862)
    Immunocytochemistry analysis of Hela cells labeling PGK1 with PGK1 Antibody (HY-P80784A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGK1 Antibody (HY-P80784A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PGK1 Antibody (YA862)
    Immunocytochemistry analysis of Hela cells labeling PGK1 with PGK1 Antibody (HY-P80784A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGK1 Antibody (HY-P80784A) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PGK1 catalyzes an ATP-producing step in glycolysis and supports tumor-cell metabolic fitness through glucose metabolism[1]. Mechanistically, mitochondrial PGK1 phosphorylates PDHK1 Thr338, activates PDHK1, inhibits the PDH complex, reduces mitochondrial pyruvate utilization, increases lactate production, and promotes brain tumorigenesis[2]. Under hypoxia or glutamine deprivation, PGK1 phosphorylates Beclin1 Ser30, enhances VPS34-Beclin1 activity, and drives stress-induced autophagy linked to glioblastoma prognosis[3]. In liver cancer models, PGK1 depletion reduced proliferation and tumorigenesis, while PGK1 acetylation promoted liver cancer progression[4]. Compared with PGK2, PGK1 represents the cytoplasmic isoform, whereas PGK2 is sperm-specific; PGK2 keeps an essentially identical active site but differs in C-terminal regions away from the active site[5]. For experimental applications, NG52 inhibited PGK1 kinase activity, restored PDH activity, reversed the Warburg effect, and suppressed glioma xenograft growth[1]. Ilicicolin H acted as a non-ATP-competitive PGK1 inhibitor that reduced glucose uptake, lactate production, and hepatocellular carcinoma proliferation[6]. Structure-based screening also identified CHR-6494 and Z57346765 as PGK1-binding inhibitors that reduced PGK1 metabolic enzyme activity and inhibited kidney renal clear cell carcinoma growth[7].- PGK1 connects glycolytic ATP production with PDHK1-PDH control and tumor metabolic remodeling[1][2]. - PGK1 differs from sperm-specific PGK2 mainly by expression context and distal structural regions[5]. - PGK1 inhibitors support mechanistic studies of glycolysis, autophagy, and cancer-cell proliferation[1][6][7].

  • Subcellular Localization

    Cytoplasm, cytosol; Mitochondrion matrix

  • Expression


    Tissue_specificity:It is primarily expressed in spermatogonia. It is located in the main sperm segment (protein level) . Its expression is significantly reduced in the testes of older men.

  • Isoforms & Post-Translational Modification

    P00558 has 2 isomers: P00558-1: 44615 Da (predicted); P00558-2: 41429 Da (predicted).
    Phosphorylated at Ser-203 by MAPK1/ERK2 under hypoxic conditions, which promotes its mitochondrial targeting

  • Subunit

    Monomer (PubMed:18463139). Interacts with kinase MAPK1/ERK2; the interaction is direct, occurs under hypoxic conditions, and promotes its interaction with PIN1 (PubMed:26942675). Interacts with peptidyl-prolyl cis-trans isomerase PIN1; the interaction is direct, occurs under hypoxic conditions, and targets the protein to the mitochondrion by promoting interactions with the TOM complex (PubMed:26942675). Interacts with mitochondrial circRNA mcPGK1 (via its 2nd stem-loop); the interaction is direct and targets the protein to the mitochondrion by promoting interactions with the TOM complex (PubMed:36849569). Interacts with pyruvate dehydrogenase kinase PDK1; the interaction is direct, occurs under hypoxic conditions and leads to PDK1-mediated inhibition of pyruvate dehydrogenase complex activity (PubMed:26942675)

  • SwissProt ID

    P00558

  • Gene ID
    5230 [NCBI]
  • Synonyms

    PGKA; MIG10; HEL-S-68p

  • Research Field

    Cardiovascular

References

[1]. Wang WL, et al. Pharmacologically inhibiting phosphoglycerate kinase 1 for glioma with NG52. Acta Pharmacol Sin. 2021 Apr;42(4):633-640. [Content Brief]

[2]. Li X, et al. Mitochondria-Translocated PGK1 Functions as a Protein Kinase to Coordinate Glycolysis and the TCA Cycle in Tumorigenesis. Mol Cell. 2016 Mar 3;61(5):705-719. [Content Brief]

[3]. Qian X, et al. Phosphoglycerate Kinase 1 Phosphorylates Beclin1 to Induce Autophagy. Mol Cell. 2017 Mar 2;65(5):917-931.e6. [Content Brief]

[4]. Hu H, et al. Acetylation of PGK1 promotes liver cancer cell proliferation and tumorigenesis. Hepatology. 2017 Feb;65(2):515-528. [Content Brief]

[5]. Sawyer GM, et al. X-ray analysis of phosphoglycerate kinase 2, a sperm-specific isoform from Mus musculus. Proteins. 2008 May 15;71(3):1134-44. [Content Brief]

[6]. Li M, et al. A novel inhibitor of PGK1 suppresses the aerobic glycolysis and proliferation of hepatocellular carcinoma. Biomed Pharmacother. 2023 Feb;158:114115. [Content Brief]

[7]. He Y, et al. Novel inhibitors targeting the PGK1 metabolic enzyme in glycolysis exhibit effective antitumor activity against kidney renal clear cell carcinoma in vitro and in vivo. Eur J Med Chem. 2024 Mar 5;267:116209. [Content Brief]

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PGK1 Antibody (YA862) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HT-29(lane 2(20ug) , NIH/3T3(lane 3(20ug) and C2C12(lane 4(20ug) using PGK1 Antibody (HY-P80784A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PGK1 with PGK1 Antibody (HY-P80784A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGK1 Antibody (HY-P80784A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PGK1 with PGK1 Antibody (HY-P80784A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGK1 Antibody (HY-P80784A) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PGK1 Antibody (YA862) Product Image
    1/16
  • Experimental Validation Results for PGK1 Antibody (YA862)
  • Experimental Validation Results for PGK1 Antibody (YA862)
  • Experimental Validation Results for PGK1 Antibody (YA862)
  • PGK1 Antibody (YA862) Product Image
  • Experimental Validation Results for PGK1 Antibody (YA862)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HT-29(lane 2(20ug) , NIH/3T3(lane 3(20ug) and C2C12(lane 4(20ug) using PGK1 Antibody (HY-P80784A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PGK1 Antibody (YA862)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PGK1 with PGK1 Antibody (HY-P80784A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGK1 Antibody (HY-P80784A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PGK1 Antibody (YA862)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PGK1 with PGK1 Antibody (HY-P80784A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGK1 Antibody (HY-P80784A) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PGK1 Antibody (YA862)
  • Experimental Validation Results for PGK1 Antibody (YA862)
  • Experimental Validation Results for PGK1 Antibody (YA862)
  • PGK1 Antibody (YA862) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HT-29(lane 2(20ug) , NIH/3T3(lane 3(20ug) and C2C12(lane 4(20ug) using PGK1 Antibody (HY-P80784A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PGK1 Antibody (YA862) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PGK1 with PGK1 Antibody (HY-P80784A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGK1 Antibody (HY-P80784A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PGK1 Antibody (YA862) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PGK1 with PGK1 Antibody (HY-P80784A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGK1 Antibody (HY-P80784A) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PGK1 Antibody (YA862) Product Image WB
  • PGK1 Antibody (YA862) Product Image ICC
  • PGK1 Antibody (YA862) Product Image ICC