BSCL2 Antibody
(Synonyms: Seipin, Bernardinelli-Seip congenital lipodystrophy type 2 protein, BSCL2)BSCL2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to BSCL2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
BSCL2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to BSCL2.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 47 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 44 kDa
Synthetic peptide corresponding to the N-terminal region of human BSCL2.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
BSCL2 mediates the formation of lipid droplets during the differentiation and maturation of adipocytes. Together with LDAF1, defines the sites of lipid droplet formation in the endoplasmic reticulum and promotes the aggregation of triacylglycerol molecules to form the core of a lipid droplet (nucleation) in the endoplasmic reticulum and drive budding of lipid droplets into the cytoplasm. BSCL2 and LDAF1 also regulate protein and lipid delivery from the endoplasmic reticulum into growing lipid droplets. During early adipogenesis, BSCL2 inhibits GPAT3 and GPAT4 activity, thereby preventing phosphatidic acid accumulation that could antagonize PPARG and block differentiation (By similarity). This inhibition is then relieved by ADIG during adipocyte maturation, allowing expansion of lipid droplets (By similarity). Regulates the maturation of ZFYVE1-positive nascent lipid droplets and the function of the RAB18-ZFYVE1 complex in mediating the formation of endoplasmic reticulum-lipid droplet contacts. Binds anionic phospholipids including phosphatidic acid[1][2][3][4][5][6][7][8].
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Subcellular Localization
Endoplasmic reticulum membrane; Lipid droplet
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Expression
Tissue_Specificity: Expressed in motor neurons in the spinal cord and cortical neurons in the frontal lobe (at protein level). Highly expressed in brain, testis and adipose tissue. -
Isoforms & Post-Translational Modification
BSCL2 has 3 isoforms, Q96G97-2: amino acid length is 398, molecular weight is 44392 Da (predicted); Q96G97-3: amino acid length is 287, molecular weight is 32654 Da (predicted); Q96G97-4: amino acid length is 462, molecular weight is 51159 Da (predicted).BSCL2 存在 3 个异构体，Q96G97-2：氨基酸个数为 398 个，分子量为 44392 Da (预测)；Q96G97-3：氨基酸个数为 287 个，分子量为 32654 Da (预测)；Q96G97-4：氨基酸个数为 462 个，分子量为 51159 Da (预测)。
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Subunit
Undecamer (an oligomer having 11 subunits) and dodecamer (an oligomer having 12 subunits) (By similarity).
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SwissProt ID
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Synonyms
Seipin, Bernardinelli-Seip congenital lipodystrophy type 2 protein, BSCL2
Documentation
[1]. Boutet E, et al. Seipin deficiency alters fatty acid Delta9 desaturation and lipid droplet formation in Berardinelli-Seip congenital lipodystrophy. Biochimie. 2009 Jun;91(6):796-803. [Content Brief]
[2]. Tian Y, et al. Tissue-autonomous function of Drosophila seipin in preventing ectopic lipid droplet formation. PLoS Genet. 2011 Apr;7(4):e1001364. [Content Brief]
[3]. Wang H, et al. Seipin is required for converting nascent to mature lipid droplets. Elife. 2016 Aug 26;5:. [Content Brief]
[4]. Yan R, et al. Human SEIPIN Binds Anionic Phospholipids. Dev Cell. 2018 Oct 22;47(2):248-256.e4. [Content Brief]
[5]. Chung J, et al. LDAF1 and Seipin Form a Lipid Droplet Assembly Complex. Dev Cell. 2019 Dec 2;51(5):551-563.e7. [Content Brief]
[6]. Salo VT, et al. Seipin regulates ER-lipid droplet contacts and cargo delivery. EMBO J. 2016 Dec 15;35(24):2699-2716. [Content Brief]
[7]. Salo VT, et al. Seipin Facilitates Triglyceride Flow to Lipid Droplet and Counteracts Droplet Ripening via Endoplasmic Reticulum Contact. Dev Cell. 2019 Aug 19;50(4):478-493.e9. [Content Brief]
[8]. Li D, et al. The ER-Localized Protein DFCP1 Modulates ER-Lipid Droplet Contact Formation. Cell Rep. 2019 Apr 9;27(2):343-358.e5. [Content Brief]
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