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Carboxypeptidase A1/A2/B Antibody (YA2486)

(Synonyms: Carboxypeptidase A1+A2+B)
Customer Review

Based on 1 Customer Validation

Carboxypeptidase A1/A2/B Antibody (YA2486) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Carboxypeptidase A1/A2/B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
  • Carboxypeptidase A1/A2/B Antibody (YA2486) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

Carboxypeptidase A1/A2/B Antibody (YA2486) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Carboxypeptidase A1/A2/B.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 44 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Carboxypeptidase A1(P15085) AA360-419 aa360-419/419.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104839

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
    Western blot analysis of extracts from U251 (lane2, 20μg) and U251 (lane2, 40μg) using Carboxypeptidase A1/A2/B Antibody (HY-P82741). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using Carboxypeptidase A1A2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using Carboxypeptidase A1A2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Carboxypeptidase A1/A2/B is a P15085: Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro. Catalyzes the conversion of leukotriene C4 to leukotriene F4 via the hydrolysis of an amide bond
    P48052: Carboxypeptidase that catalyzes the release of a C-terminal amino acid, with a preference for large aromatic C-terminal residues
    [1][2].

  • Subcellular Localization

    P15085: Secreted
    P15086: Secreted; Zymogen granule lumen
    P48052: Secreted

  • Expression


    Tissue_specificity: P15086: Pancreas

  • Isoforms & Post-Translational Modification

    Human (P15085): 419 amino acids, molecular weight 47,140 Da.
    Human (P15086): 417 amino acids, molecular weight 47,368 Da.
    Human (P48052): 419 amino acids, molecular weight 47,030 Da.

  • Subunit

    P15085: Monomer. May form a complex with proelastase 2

  • SwissProt ID

    P15085 / P15086 / P48052

  • Gene ID
    1357 [NCBI] / 1360 [NCBI] / 1358 [NCBI]
  • Synonyms

    Carboxypeptidase A1+A2+B

  • Research Field

    -

Carboxypeptidase A1/A2/B Antibody (YA2486) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from U251 (lane2, 20μg) and U251 (lane2, 40μg) using Carboxypeptidase A1/A2/B Antibody (HY-P82741). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using Carboxypeptidase A1A2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using Carboxypeptidase A1A2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Carboxypeptidase A1/A2/B Antibody (YA2486) Product Image
    1/16
  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
  • Carboxypeptidase A1/A2/B Antibody (YA2486) Product Image
  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from U251 (lane2, 20μg) and U251 (lane2, 40μg) using Carboxypeptidase A1/A2/B Antibody (HY-P82741). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using Carboxypeptidase A1A2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using Carboxypeptidase A1A2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
  • Experimental Validation Results for Carboxypeptidase A1/A2/B Antibody (YA2486)
  • Carboxypeptidase A1/A2/B Antibody (YA2486) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from U251 (lane2, 20μg) and U251 (lane2, 40μg) using Carboxypeptidase A1/A2/B Antibody (HY-P82741). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Carboxypeptidase A1/A2/B Antibody (YA2486) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using Carboxypeptidase A1A2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Carboxypeptidase A1/A2/B Antibody (YA2486) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using Carboxypeptidase A1A2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Carboxypeptidase A1/A2/B Antibody (YA2486) Product Image WB
  • Carboxypeptidase A1/A2/B Antibody (YA2486) Product Image IHC-P
  • Carboxypeptidase A1/A2/B Antibody (YA2486) Product Image IHC-P