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  5. Carboxypeptidase A1/A2/B Antibody (YA2486)

Carboxypeptidase A1/A2/B Antibody (YA2486)

Cat. No.: HY-P82741
COA
SDS
User Guide for Antibodies Technical Support

Carboxypeptidase A1/A2/B Antibody (YA2486) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Carboxypeptidase A1/A2/B.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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제품 설명

Carboxypeptidase A1/A2/B Antibody (YA2486) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Carboxypeptidase A1/A2/B.
Host

Rabbit
Clonality

Recombinant,Monoclonal
분자량
Predicted band size: 47 kDa;
Observed band size: 44 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Carboxypeptidase A1(P15085) AA360-419 aa360-419/419.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
IHC-P

  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using Carboxypeptidase A1A2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using Carboxypeptidase A1A2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background
Function：P15085: Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro (PubMed:20385563, PubMed:8806703). Catalyzes the conversion of leukotriene C4 to leukotriene F4 via the hydrolysis of an amide bond (By similarity)
P48052: Carboxypeptidase that catalyzes the release of a C-terminal amino acid, with a preference for large aromatic C-terminal residues
Subcellular Localization：P15085: Secreted
P15086: Secreted; Zymogen granule lumen
P48052: Secreted
Expression：
Tissue_specificity: P15086: Pancreas
Isoforms & Post-Translational Modification：Human (P15085): 419 amino acids, molecular weight 47,140 Da.
Human (P15086): 417 amino acids, molecular weight 47,368 Da.
Human (P48052): 419 amino acids, molecular weight 47,030 Da.
Subunit：P15085: Monomer. May form a complex with proelastase 2
RRID
Database

Entrez Gene: 1357 Human ; 1360 Human ; 1358 Human

SwissProt: P15085 Human ; P15086 Human ; P48052

Research Field

-
Synonyms
Carboxypeptidase A1+A2+B
각종 서류
SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Carboxypeptidase A1/A2/B Antibody (YA2486) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
Carboxypeptidase A1/A2/B Antibody (YA2486)
Cat. No.:
HY-P82741
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