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Retinoid X Receptor alpha Antibody (YA095)

(Synonyms: NR2B1, RXRA, Retinoic acid receptor RXR-alpha, Nuclear receptor subfamily 2 group B member 1, Retinoid X receptor alpha)
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Based on 1 publication(s) in Google Scholar

Retinoid X Receptor alpha Antibody (YA095) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Retinoid X Receptor alpha.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL ICC IHC-P
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
  • Retinoid X Receptor alpha Antibody (YA095) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:2000 1:50-1:400 1:50 Use at an assay dependent concentration.

Product Details

Description

Retinoid X Receptor alpha Antibody (YA095) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Retinoid X Receptor alpha.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 51 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 51 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Retinoid X Receptor alpha.AA range:1-50.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102417

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
    Immunocytochemistry analysis of HCT116 cells labeling Retinoid X Receptor alpha with Retinoid X Receptor alpha Antibody (HY-P80309) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Retinoid X Receptor alpha Antibody (HY-P80309) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
    Immunocytochemistry analysis of MCF-7 cells labeling Retinoid X Receptor alpha with Retinoid X Receptor alpha Antibody (HY-P80309) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Retinoid X Receptor alpha Antibody (HY-P80309)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
    Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Retinoid X Receptor alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80309, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
    Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Retinoid X Receptor alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80309, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    The retinoid X receptor (RXR) belongs to the steroid/thyroid hormone superfamily of nuclear receptors (NRs) and includes RXRα, β, and γ (NR2B1, NR2B2 and NR2B3) . They feature a modular structure, which can be dissected in several domains with associated f

  • Subcellular Localization

    Nucleus; Cytoplasm; Mitochondrion

  • Expression


    Tissue_specificity:Expressed in lung fibroblasts (at protein level) (PubMed:30216632) . Expressed in monocytes (PubMed:26463675) . Highly expressed in liver, also found in kidney and brain (PubMed:14702039, PubMed:2159111, PubMed:24275569)

    Induction:Down-regulated by aging (PubMed:26463675) . Induced by pulsatile shear stress (PubMed:28167758)

  • Isoforms & Post-Translational Modification

    P19793 has 2 isomers: P19793-1: 50811 Da (predicted); P19793-2: 41060 Da (predicted).
    Acetylated by EP300; acetylation enhances DNA binding and transcriptional activity;Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain (By similarity). Constitutively phosphorylated on Ser-21 in the presence or absence of ligand (By similarity). Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA (PubMed:11162439). This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA (PubMed:11162439);Ubiquitinated by UBR5, leading to its degradation: UBR5 specifically recognizes and binds ligand-bound RXRA when it is not associated with coactivators (NCOAs) (PubMed:37478846). In presence of NCOAs, the UBR5-degron is not accessible, preventing its ubiquitination and degradation (PubMed:37478846);Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6

  • Subunit

    Homodimer (PubMed:10669605, PubMed:17761950). Heterodimer (via C-terminus) with RARA; required for ligand-dependent retinoic acid receptor transcriptional activity; association with RARA is enhanced by pulsatile shear stress (PubMed:10698945, PubMed:15509776, PubMed:28167758). Heterodimer with PPARA (via the leucine-like zipper in the LBD); the interaction is required for PPARA transcriptional activity (PubMed:10195690, PubMed:11698662, PubMed:11915042). Heterodimerizes with PPARG (PubMed:10882139, PubMed:11698662). Heterodimerizes (via NR LBD) with RARB (PubMed:29021580). Heterodimerizes with NR1H4; the heterodimerization enhances the binding affinity for LXXLL motifs from coactivators (PubMed:30275017). Interacts with NCOA3 and NCOA6 coactivators (PubMed:10567404, PubMed:9267036). Interacts with coactivator FAM120B (By similarity). Interacts with coactivator PELP1, SENP6, SFPQ, DNTTIP2 and RNF8 (PubMed:11259580, PubMed:14981089, PubMed:15047147, PubMed:16574651, PubMed:16912044). Interacts with PRMT2 (PubMed:12039952). Interacts with ASXL1 (By similarity). Interacts with BHLHE40/DEC1, BHLHE41/DEC2, NCOR1 and NCOR2 (PubMed:19786558). Interacts in a ligand-dependent fashion with MED1 and NCOA1 (PubMed:10882139, PubMed:11698662, PubMed:19786558). Interacts with VDR (PubMed:28698609). Interacts with EP300; the interaction is decreased by 9-cis retinoic acid (PubMed:17761950). Heterodimer (via C-terminus) with NR4A1 (via DNA-binding domain); DNA-binding of the heterodimer is enhanced by 9-cis retinoic acid (PubMed:15509776, PubMed:17761950). NR4A1 competes with EP300 for interaction with RXRA and thereby attenuates EP300 mediated acetylation of RXRA (PubMed:17761950). In the absence of hormonal ligand, interacts with TACC1 (PubMed:20078863). Interacts ith IGFBP3 (PubMed:10874028)

  • SwissProt ID

    P19793

  • Gene ID
    6256 [NCBI]
  • Synonyms

    NR2B1, RXRA, Retinoic acid receptor RXR-alpha, Nuclear receptor subfamily 2 group B member 1, Retinoid X receptor alpha

  • Research Field

    Epigenetics and Nuclear Signaling

Retinoid X Receptor alpha Antibody (YA095) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HCT116 cells labeling Retinoid X Receptor alpha with Retinoid X Receptor alpha Antibody (HY-P80309) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Retinoid X Receptor alpha Antibody (HY-P80309) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Retinoid X Receptor alpha with Retinoid X Receptor alpha Antibody (HY-P80309) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Retinoid X Receptor alpha Antibody (HY-P80309)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Retinoid X Receptor alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80309, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Retinoid X Receptor alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80309, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Retinoid X Receptor alpha Antibody (YA095) Product Image
    1/16
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
  • Retinoid X Receptor alpha Antibody (YA095) Product Image
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HCT116 cells labeling Retinoid X Receptor alpha with Retinoid X Receptor alpha Antibody (HY-P80309) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Retinoid X Receptor alpha Antibody (HY-P80309) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Retinoid X Receptor alpha with Retinoid X Receptor alpha Antibody (HY-P80309) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Retinoid X Receptor alpha Antibody (HY-P80309)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Retinoid X Receptor alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80309, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Retinoid X Receptor alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80309, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
  • Experimental Validation Results for Retinoid X Receptor alpha Antibody (YA095)
  • Retinoid X Receptor alpha Antibody (YA095) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HCT116 cells labeling Retinoid X Receptor alpha with Retinoid X Receptor alpha Antibody (HY-P80309) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Retinoid X Receptor alpha Antibody (HY-P80309) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Retinoid X Receptor alpha Antibody (YA095) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Retinoid X Receptor alpha with Retinoid X Receptor alpha Antibody (HY-P80309) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Retinoid X Receptor alpha Antibody (HY-P80309)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Retinoid X Receptor alpha Antibody (YA095) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Retinoid X Receptor alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80309, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Retinoid X Receptor alpha Antibody (YA095) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Retinoid X Receptor alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80309, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Retinoid X Receptor alpha Antibody (YA095) Product Image ICC
  • Retinoid X Receptor alpha Antibody (YA095) Product Image ICC
  • Retinoid X Receptor alpha Antibody (YA095) Product Image IHC-P
  • Retinoid X Receptor alpha Antibody (YA095) Product Image IHC-P