ENDOG Antibody
(Synonyms: Endo G, ENDOG)ENDOG Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ENDOG.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
ENDOG Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ENDOG.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 33 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 32 kDa
Synthetic peptide corresponding to the center region of human ENDOG.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ENDOG is an Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA. The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs (By similarity). Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs (By similarity). Promotes conservative recombination when the DNA is 5hmC-modified. Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response. GSK3-beta mediated phosphorylation of ENDOG enhances its interaction with YWHAG, leading to the release of TSC2 and PIK3C3 from YWHAG resulting in mTOR pathway suppression and autophagy initiation. Promotes cleavage of mtDNA in response to oxidative and nitrosative stress, in turn inducing compensatory mtDNA replication[1][2][3].
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Subcellular Localization
Mitochondrion
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Isoforms & Post-Translational Modification
ENDOG has an amino acid length of 297, molecular weight is 32620 Da.GSK3-β 介导的 Thr-128 和 Ser-288 位点双重磷酸化是其与 YWHAG 相互作用及诱导自噬的必要条件ENDOG 的氨基酸个数为 297 个，分子量为 32620 Da。GSK3-beta-mediated dual phosphorylations at Thr-128 and Ser-288 is necessary for its interaction with YWHAG and the induction of autophagy
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Subunit
Homodimer; disulfide-linked (By similarity).
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SwissProt ID
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Synonyms
Endo G, ENDOG
Documentation
[1]. Robertson AB, et al. Endonuclease G preferentially cleaves 5-hydroxymethylcytosine-modified DNA creating a substrate for recombination. Nucleic Acids Res. 2014 Dec 1;42(21):13280-93. [Content Brief]
[2]. Wang W, et al. Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response. Nat Commun. 2021 Jan 20;12(1):476. [Content Brief]
[3]. Wiehe RS, et al. Endonuclease G promotes mitochondrial genome cleavage and replication. Oncotarget. 2018 Apr 6;9(26):18309-18326. [Content Brief]
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