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gamma Adaptin Antibody (YA2993)

(Synonyms: ADTG; AP1G1; CLAPG1)
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Based on 1 Customer Validation

gamma Adaptin Antibody (YA2993) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to gamma Adaptin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
  • gamma Adaptin Antibody (YA2993) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

gamma Adaptin Antibody (YA2993) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to gamma Adaptin.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 91 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human AP1G1 aa780-822.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105362

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
    Western blot analysis of extracts from HEK293T(lane 2(20ug) ,Jurkat(lane 3(20ug) and HepG2(lane 4(20ug) using gamma Adaptin Antibody (HY-P83248) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using gamma Adaptin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using gamma Adaptin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    gamma Adaptin is a Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. In association with AFTPH/aftiphilin in the aftiphilin/p200/gamma-synergin complex, involved in the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes[1].

  • Subcellular Localization

    Golgi apparatus; Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm; Cytoplasm, perinuclear region; Cytoplasmic vesicle, clathrin-coated vesicle; Membrane, clathrin-coated pit

  • Expression


    Tissue_specificity:Broad expression

  • Isoforms & Post-Translational Modification

    O43747 has 2 isomers: O43747-1: 91351 Da (predicted); O43747-2: 91723 Da (predicted).

  • Subunit

    Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3) (PubMed:34102099).

  • SwissProt ID

    O43747

  • Gene ID
    164 [NCBI]
  • Synonyms

    ADTG; AP1G1; CLAPG1

  • Research Field

    -

gamma Adaptin Antibody (YA2993) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HEK293T(lane 2(20ug) ,Jurkat(lane 3(20ug) and HepG2(lane 4(20ug) using gamma Adaptin Antibody (HY-P83248) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using gamma Adaptin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using gamma Adaptin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • gamma Adaptin Antibody (YA2993) Product Image
    1/16
  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
  • gamma Adaptin Antibody (YA2993) Product Image
  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HEK293T(lane 2(20ug) ,Jurkat(lane 3(20ug) and HepG2(lane 4(20ug) using gamma Adaptin Antibody (HY-P83248) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using gamma Adaptin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using gamma Adaptin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
  • Experimental Validation Results for gamma Adaptin Antibody (YA2993)
  • gamma Adaptin Antibody (YA2993) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HEK293T(lane 2(20ug) ,Jurkat(lane 3(20ug) and HepG2(lane 4(20ug) using gamma Adaptin Antibody (HY-P83248) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • gamma Adaptin Antibody (YA2993) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using gamma Adaptin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • gamma Adaptin Antibody (YA2993) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using gamma Adaptin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • gamma Adaptin Antibody (YA2993) Product Image WB
  • gamma Adaptin Antibody (YA2993) Product Image IHC-P
  • gamma Adaptin Antibody (YA2993) Product Image IHC-P