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CD20 Antibody (YA6136)

(Synonyms: B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD antigen CD20; )
Customer Review

Based on 1 Customer Validation

CD20 Antibody (YA6136) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD20.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for CD20 Antibody (YA6136)
  • Experimental Validation Results for CD20 Antibody (YA6136)
  • Experimental Validation Results for CD20 Antibody (YA6136)
  • Experimental Validation Results for CD20 Antibody (YA6136)
  • CD20 Antibody (YA6136) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:5000 1:1000-1:5000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

CD20 Antibody (YA6136) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD20.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719158

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD20 Antibody (YA6136)
    Western blot analysis of extracts from Hela (lane2(20μg), Raji (lane3(20μg), Ramos (lane4(20μg) and Daudi (lane5(20μg) using CD20 Antibody (HY-P86444). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD20 Antibody (YA6136)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD20 Antibody (HY-P86444, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD20 Antibody (YA6136)
    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using CD20 Antibody (HY-P86444, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD20 Antibody (YA6136)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using CD20 Antibody (HY-P86444, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    CD20 is a 33-37 kDa non-glycosylated protein expressed specifically on the surface of normal and malignant B lymphocytes, and belongs to the MS4A (membrane-spanning 4-domain family A) protein family. CD20 is a general B-cell marker expressed by the majority of B cells starting from late pre-B lymphocytes (it is not expressed by pro-B lymphocytes), and its expression is lost in terminally differentiated plasmablasts and plasma cells. Early experiments suggested that it was important in the regulation of human B-cell activation, proliferation, and Ca2+ transport. However, CD20-deficient mice appeared normal. Hence, the exact biological function of CD20 and its physiological ligand remain to be fully defined.240 Regardless of the uncertainty in the biological role of this antigen, it is a good target for cancer immunotherapy.

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Cell membrane; Lipid-anchor

  • Expression


    Tissue_specificity:Expressed on B cells

  • Isoforms & Post-Translational Modification

    CD20 has 2 isomers: P11836-1: 33077 Da (predicted); P11836-2: 14581 Da (predicted).
    Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate

  • Subunit

    Forms homotetramers (PubMed:18474602, PubMed:7684739). Interacts with the heavy and light chains of cell surface IgM, the antigen-binding components of the BCR (PubMed:18474602)

  • SwissProt ID

    P11836

  • Gene ID
    931 [NCBI]
  • Synonyms

    B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD antigen CD20;

CD20 Antibody (YA6136) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), Raji (lane3(20μg), Ramos (lane4(20μg) and Daudi (lane5(20μg) using CD20 Antibody (HY-P86444). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD20 Antibody (HY-P86444, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using CD20 Antibody (HY-P86444, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using CD20 Antibody (HY-P86444, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD20 Antibody (YA6136) Product Image
    1/16
  • Experimental Validation Results for CD20 Antibody (YA6136)
  • Experimental Validation Results for CD20 Antibody (YA6136)
  • Experimental Validation Results for CD20 Antibody (YA6136)
  • Experimental Validation Results for CD20 Antibody (YA6136)
  • CD20 Antibody (YA6136) Product Image
  • Experimental Validation Results for CD20 Antibody (YA6136)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), Raji (lane3(20μg), Ramos (lane4(20μg) and Daudi (lane5(20μg) using CD20 Antibody (HY-P86444). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD20 Antibody (YA6136)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD20 Antibody (HY-P86444, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD20 Antibody (YA6136)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using CD20 Antibody (HY-P86444, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD20 Antibody (YA6136)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using CD20 Antibody (HY-P86444, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD20 Antibody (YA6136)
  • Experimental Validation Results for CD20 Antibody (YA6136)
  • Experimental Validation Results for CD20 Antibody (YA6136)
  • Experimental Validation Results for CD20 Antibody (YA6136)
  • CD20 Antibody (YA6136) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2(20μg), Raji (lane3(20μg), Ramos (lane4(20μg) and Daudi (lane5(20μg) using CD20 Antibody (HY-P86444). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • CD20 Antibody (YA6136) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD20 Antibody (HY-P86444, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD20 Antibody (YA6136) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using CD20 Antibody (HY-P86444, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD20 Antibody (YA6136) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using CD20 Antibody (HY-P86444, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD20 Antibody (YA6136) Product Image WB
  • CD20 Antibody (YA6136) Product Image IHC-P
  • CD20 Antibody (YA6136) Product Image IHC-P
  • CD20 Antibody (YA6136) Product Image IHC-P