CLIP170 Antibody (YA2810)(PBS only)

Cat. No.: HY-P83065A: Data Sheet User Guide for Antibodies
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CLIP170 Antibody (YA2810) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CLIP170.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CLIP170 Antibody (YA2810) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CLIP170.

Host

Rabbit

Molecular Weight

Predicted band size: 162 kDa;

Observed band size: 170 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P30622

Gene ID

6249 [NCBI]

Immunogen

A synthetic peptide of human CLIP170

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking

Subcellular Localization：Cytoplasm; Cytoplasm, cytoskeleton; Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, ruffle

Expression：
Tissue_specificity:Detected in dendritic cells (at protein level) . Highly expressed in the Reed-Sternberg cells of Hodgkin disease

Isoforms & Post-Translational Modification：P30622 has 3 isomers: P30622-3: 162246 Da (predicted); P30622-1: 160990 Da (predicted); P30622-2: 156781 Da (predicted).
Phosphorylated. Phosphorylation induces conformational changes by increasing the affinity of the N-terminus for C-terminus, resulting in inhibition of its function thus decreasing its binding to microtubules and DCTN1. Exhibits a folded, autoinhibited conformation when phosphorylated and an open conformation when dephosphorylated with increased binding affinity to microtubules and DCTN1. Phosphorylation regulates its recruitment to tyrosinated microtubules and the recruitment of vesicular cargo to microtubules in neurons (By similarity). Phosphorylation by MTOR may positively regulate CLIP1 association with microtubules (PubMed:12231510)

Subunit：Interacts with MTOR; phosphorylates and regulates CLIP1 (PubMed:12231510). Interacts (via CAP-Gly domains) with tubulin (PubMed:17563362, PubMed:17889670). Interacts with SLAIN2 (PubMed:21646404).

Research Field

Cell Biology

Synonyms

RSN; CLIP; CYLN1; CLIP170; CLIP-170

Documentation

User Guide for Antibodies (1077 KB)