SMYD3 Antibody (YA2003)(PBS only)

Cat. No.: HY-P82258A: Data Sheet User Guide for Antibodies
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SMYD3 Antibody (YA2003) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMYD3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SMYD3 Antibody (YA2003) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMYD3.

Host

Rabbit

Molecular Weight

Predicted band size: 49 kDa;

Observed band size: 49 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9H7B4

Gene ID

64754 [NCBI]

Immunogen

A synthetic peptide of human SMYD3

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation (PubMed:15235609, PubMed:22419068). Also methylates 'Lys-5' of histone H4 (PubMed:22419068). Plays an important role in transcriptional activation as a member of an RNA polymerase complex (PubMed:15235609). Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences (PubMed:15235609)

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas

Isoforms & Post-Translational Modification：Q9H7B4 has 3 isomers: Q9H7B4-1: 49097 Da (predicted); Q9H7B4-2: 29759 Da (predicted); Q9H7B4-3: 42625 Da (predicted).

Subunit：Interacts with HSPCA (PubMed:15235609). Interacts with HELZ (PubMed:15235609). Interacts with POLR2A; the interaction may be indirect and may be mediated by HELZ (PubMed:15235609). Interacts with HSP90AA1; this interaction enhances SMYD3 histone-lysine N-methyltransferase (PubMed:25738358)

Research Field

Epigenetics and Nuclear Signaling

Synonyms

KMT3E; ZMYND1; ZNFN3A1; bA74P14.1

Documentation

User Guide for Antibodies (1077 KB)