Cathepsin D Antibody (YA550)
(Synonyms: CPSD, CTSD, Cathepsin D)
Based on 1 Customer Validation
Cathepsin D Antibody (YA550) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cathepsin D.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IHC-P, IP
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Reactivity :
Human, Mouse
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Formulation:
Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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| Dilution Ratio | 1:500-1:2000 | 1:100-1:500 | 1:100-1:500 | Use at an assay dependent concentration. |
Product Details
Cathepsin D Antibody (YA550) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cathepsin D.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 45/28/52kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 45/28/52 kDa
Synthetic peptide corresponding to Human Cathepsin D.AA range:363-412.
Endogenous
Protein A affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from MCF-7 (lane2(20μg), SK-BR-3 (lane3(20μg), HepG2 (lane4(20μg) and A431 (lane5(20μg) using Cathepsin D Antibody (HY-P80384). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Cathepsin D Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80384, 1/3000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Cathepsin D Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80384, 1/3000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of Hela cells labeling Cathepsin D with Cathepsin D Antibody (HY-P80384) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cathepsin D Antibody (HY-P80384)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Background
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Function
Cathepsin D is a lysosomal aspartic protease whose major described function is intracellular catabolism in lysosomal compartments, with additional roles in hormone processing and antigen processing[1]. Its function extends beyond nonspecific acidic bulk degradation, because cathepsin D-deficient mice maintain lysosomal bulk proteolysis but develop intestinal mucosal atrophy, lymphoid-cell destruction, and early death, indicating roles in limited proteolysis and tissue homeostasis[2]. Mechanistically, cathepsin D participates in endocytic and autophagic pathways where lysosomal proteases regulate substrate degradation and cellular remodeling[3]. In apoptosis-related models, mature lysosomal cathepsin D can translocate to the cytosol, and cathepsin D activity has been linked to induced apoptosis[4]. In breast cancer biology, cathepsin D is overexpressed and hypersecreted by epithelial breast cancer cells, correlates with clinical metastasis, and stimulates tumorigenicity, metastasis, cancer-cell proliferation, fibroblast outgrowth, and angiogenesis[4]. Compared with cathepsin E, cathepsin D showed distinct cytosolic activity after lysosomal permeabilization, because cathepsin D silencing prevented stefin B degradation whereas cathepsin E silencing did not[5]. For experimental applications, pepstatin A inhibited aspartic-protease-dependent stefin B degradation, and fluorogenic peptide probes detected cathepsin D activity in macrophages challenged with bacteria[5][6].
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Subcellular Localization
Lysosome; Melanosome; Secreted, extracellular space
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Expression
Tissue_specificity:Expression in the extracellular space of aortic cells (protein level) (PubMed: 20551380) . Expression in the liver (protein level) (PubMed: 1426530) . -
Subunit
Consists of a light chain and a heavy chain (PubMed:1426530, PubMed:8393577). Interacts with ADAM30; this leads to activation of CTSD (PubMed:27333034).
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SwissProt ID
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Synonyms
CPSD, CTSD, Cathepsin D
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Research Field
Neuroscience
Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
[1]. Fusek M, et al. Dual role of cathepsin D: ligand and protease. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2005 Jun;149(1):43-50. [Content Brief]
[2]. Saftig P, et al. Mice deficient for the lysosomal proteinase cathepsin D exhibit progressive atrophy of the intestinal mucosa and profound destruction of lymphoid cells. EMBO J. 1995 Aug 1;14(15):3599-608. [Content Brief]
[3]. Müller S, et al. Specific functions of lysosomal proteases in endocytic and autophagic pathways. Biochim Biophys Acta. 2012 Jan;1824(1):34-43. [Content Brief]
[4]. Liaudet-Coopman E, et al. Cathepsin D: newly discovered functions of a long-standing aspartic protease in cancer and apoptosis. Cancer Lett. 2006 Jun 18;237(2):167-79. [Content Brief]
[5]. Železnik TZ, et al. Aspartic cathepsin D degrades the cytosolic cysteine cathepsin inhibitor stefin B in the cells. Biochem Biophys Res Commun. 2015 Sep 18;465(2):213-7. [Content Brief]
[6]. Rodriguez-Rios M, et al. A fluorogenic, peptide-based probe for the detection of Cathepsin D in macrophages. Commun Chem. 2023 Nov 2;6(1):237. [Content Brief]
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