NM23A Antibody (YA4991)

Cat. No.: HY-P85299: Ficha de datos User Guide for Antibodies
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NM23A Antibody (YA4991) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to NM23A.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

NM23A Antibody (YA4991) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to NM23A.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 17 kDa;

Species Reactivity

Human

SwissProt ID

P15531

Gene ID

4830 [NCBI]

Immunogen

Recombinant human NME1 (His-tagged). The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-500

Sensitivity	Endogenous	Pureza	affinity purified by Protein G
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Isomer 1 is expressed in the heart, brain, placenta, lungs, liver, skeletal muscle, pancreas, spleen, and thymus. It is expressed in lung cancer cell lines but not in normal lung tissue. Isomer 2 is universally expressed, and its expression is also associated with tumor differentiation.

Isoforms & Post-Translational Modification：P15531 has 3 isomers: P15531-1: 17149 Da (predicted); P15531-2: 19654 Da (predicted); P22392-2: 30137 Da (predicted).

Subunit：Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with PRUNE1. Component of the SET complex, composed of at least ANP32A, APEX1, HMGB2, NME1, SET and TREX1.

Synonyms

NME1; NDPKA; NM23; Nucleoside diphosphate kinase A; NDK A; NDP kinase A; Granzyme A-activated DNase; GAAD; Metastasis inhibition factor nm23; Tumor metastatic process-associated protein; nm23-H1

Documentación

User Guide for Antibodies (1077 KB)