BCAT1 Antibody (YA7813)

Cat. No.: HY-P88129: Ficha de datos COA
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BCAT1 Antibody (YA7813) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to BCAT1.

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Tamaño	Stock	Cantidad
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn
Referencias

Descripciòn

BCAT1 Antibody (YA7813) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to BCAT1.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 42.8 kDa

Species Reactivity

Human, Dog, Mouse

SwissProt ID

P54687

Immunogen

Full length human recombinant protein of human BCAT1 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Pureza	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Jurkat (lane2(20μg), Hela (lane3(20μg),Neuro-2a (lane4(20μg) and HepG2 (lane5(20μg) using BCAT1 Antibody (HY-P88129). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

Background

Function：Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine

Subcellular Localization：Cytoplasm

Expression：
Tissue_Specificity: During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma

Isoforms & Post-Translational Modification：P54687 has 5 isomers: P54687-1: 42966 Da (predicted); P54687-2: 36298 Da (predicted); P54687-3: 38645 Da (predicted); P54687-4: 42838 Da (predicted); P54687-5: 44131 Da (predicted).

Subunit：Homodimer

Synonyms

BCATC; BCT1; ECA39; MECA39; PNAS121; PP18

Documentación

Select Batch:

Ficha de datos (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/huvec-cell.html [Content Brief]