BCAT1 Antibody (YA7813)
(Synonyms: BCATC; BCT1; ECA39; MECA39; PNAS121; PP18)
Based on 1 Customer Validation
BCAT1 Antibody (YA7813) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to BCAT1.
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Host:
Mouse
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Isotype:
IgG2a
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Application:
WB, IHC-P
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Reactivity :
Human, Dog, Mouse
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:150-500 |
Product Details
BCAT1 Antibody (YA7813) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to BCAT1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Dog, Mouse
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Calculated Molecular Weight Predicted band size: 42.8 kDa
Full length human recombinant protein of human BCAT1 produced in HEK293T cell.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG2a
Product Properties
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Appearance
Solution
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Jurkat (lane2(20μg), Hela (lane3(20μg),Neuro-2a (lane4(20μg) and HepG2 (lane5(20μg) using BCAT1 Antibody (HY-P88129). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human glioma tissue using BCAT1 Antibody (HY-P88129, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using BCAT1 Antibody (HY-P88129, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using BCAT1 Antibody (HY-P88129, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using BCAT1 Antibody (HY-P88129, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Background
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Function
BCAT1 catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine
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Subcellular Localization
Cytoplasm
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Expression
Tissue_Specificity: During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma -
Isoforms & Post-Translational Modification
P54687 has 5 isomers: P54687-1: 42966 Da (predicted); P54687-2: 36298 Da (predicted); P54687-3: 38645 Da (predicted); P54687-4: 42838 Da (predicted); P54687-5: 44131 Da (predicted).
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Subunit
Homodimer
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SwissProt ID
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Synonyms
BCATC; BCT1; ECA39; MECA39; PNAS121; PP18
Documentation
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