WNK1 Antibody (YA2213)

(Synonyms: WNK1; HSN2; KDP; KIAA0344; PRKWNK1; Serine/threonine-protein kinase WNK1; Erythrocyte 65 kDa protein; p65; Kinase deficient protein; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; hWNK1)
Customer Review

Based on 1 Customer Validation

WNK1 Antibody (YA2213) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to WNK1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-1:2000 1:50-1:100

Product Details

Description

WNK1 Antibody (YA2213) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to WNK1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 251 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 251 kDa
Immunogen

A synthesized peptide derived from human WNK1 aa50-100/2382.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for WNK1 Antibody (YA2213)
    Immunohistochemical analysis of paraffin-embedded human thyroid cancer using WNK1 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82468, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for WNK1 Antibody (YA2213)
    Immunohistochemical analysis of paraffin-embedded human prostate cancer using WNK1 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82468, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for WNK1 Antibody (YA2213)
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer using WNK1 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82468, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for WNK1 Antibody (YA2213)
    Immunohistochemical analysis of paraffin-embedded human cervical cancer using WNK1 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82468, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for WNK1 Antibody (YA2213)
    Immunohistochemical analysis of paraffin-embedded human tonsil using WNK1 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82468, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for WNK1 Antibody (YA2213)
    Immunohistochemical analysis of paraffin-embedded human kidney using WNK1 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82468, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    WNK1 is a serine/threonine kinase that regulates ion balance by activating SPAK/OSR1 signaling, which phosphorylates cation-chloride cotransporters including NKCC1, NKCC2, and NCC[1]. Mechanistically, WNK1 and WNK4 phosphorylate and activate SPAK/OSR1, placing WNK1 within a conserved WNK-SPAK/OSR1 transporter-control pathway[2]. In disease models, WNK1 or WNK4 mutations cause pseudohypoaldosteronism type II/familial hyperkalemic hypertension, linking pathway activation to renal salt handling, hyperkalemia, and hypertension[3]. Compared with WNK4, WNK1-driven familial hyperkalemic hypertension remains SPAK-dependent but shows different distal-nephron effects on NCC abundance and phosphorylation[4]. For experimental applications, WNK463 provides a pan-WNK inhibitor tool that affects cardiovascular, renal, blood-pressure, fluid, and electrolyte phenotypes in rodent hypertension models[5].

  • Subcellular Localization

    Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, spindle

  • Expression


    Tissue_specificity:Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle; Strong expression in dorsal root ganglia and spinal cord; This isoform is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron

  • Isoforms & Post-Translational Modification

    Q9H4A3 has 6 isomers: Q9H4A3-1: 250794 Da (predicted); Q9H4A3-2: 225560 Da (predicted); Q9H4A3-4: 206646 Da (predicted); Q9H4A3-5: 279538 Da (predicted); Q9H4A3-6: 279713 Da (predicted); Q9H4A3-7: 299725 Da (predicted).
    Autophosphorylated at Ser-378 and Ser-382, promoting its activity (PubMed:15883153, PubMed:16263722, PubMed:16832045, PubMed:17190791, PubMed:31656913, PubMed:34289367). Autophosphorylation at Ser-382 is inhibited by intracellular calcium (By similarity). Phosphorylation at Thr-60 increases ability to activate SGK1 (By similarity);Ubiquitinated by the BCR(KLHL3) complex, leading to its degradation (PubMed:23387299, PubMed:23576762). Also ubiquitinated by the BCR(KLHL2) complex (PubMed:23838290);May be O-glycosylated

  • Subunit

    Interacts with WNK3 (By similarity). Interacts with WNK4; inhibiting the activity of WNK4 (By similarity). Interacts with SGK1; promoting its activation (PubMed:36373794). Associates with the mTORC2 complex (PubMed:36373794). Interacts with UVRAG (PubMed:27911840). Interacts (via amphipathic alpha-helix region) with EMC2; promoting the ER membrane protein complex assembly (PubMed:33964204)

  • SwissProt ID

    Q9H4A3

  • Gene ID
  • Synonyms

    WNK1; HSN2; KDP; KIAA0344; PRKWNK1; Serine/threonine-protein kinase WNK1; Erythrocyte 65 kDa protein; p65; Kinase deficient protein; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; hWNK1

  • Research Field

    Signal Transduction

References

WNK1 Antibody (YA2213) Related Classifications

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Minimum order quantity
100 mg

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