SPAM1 Antibody (YA5946)
(Synonyms: SPAM1; HYAL3; PH20; Hyaluronidase PH-20 ; Hyal-PH20; Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20; )
Based on 1 Customer Validation
SPAM1 Antibody (YA5946) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SPAM1.
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Host:
Rabbit
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Isotype:
IgG/Kappa
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Application:
WB, IHC-P
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Reactivity :
Human
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-5000 | 1:100-300 |
Product Details
SPAM1 Antibody (YA5946) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SPAM1.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 56 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Protein A
Non-conjugated
Unmodified
IgG/Kappa
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Raji (lane 1(20μg)) 、NCCIT (lane 2(20μg)) 、SH-SY5Y (lane 3(20μg)) and PC-3 (lane 4(20μg)) using SPAM1 Antibody (HY-P86254) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Background
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Function
SPAM1 is involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid
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Subcellular Localization
Cell membrane; Lipid-anchor, GPI-anchor
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Expression
Tissue_specificity:Testis -
Isoforms & Post-Translational Modification
P38567 has 2 isomers: P38567-1: 57848 Da (predicted); P38567-2: 58395 Da (predicted).
N-glycosylated -
SwissProt ID
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Synonyms
SPAM1; HYAL3; PH20; Hyaluronidase PH-20 ; Hyal-PH20; Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20;
Documentation
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