Olig2 Antibody(YA6613)

Cat. No.: HY-P86920: Data Sheet COA
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Olig2 Antibody(YA6613) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Olig2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Olig2 Antibody(YA6613) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Olig2.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 32 kDa;

Observed band size: 36 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey, Pig

SwissProt ID

Q13516

Gene ID

10215 [NCBI]

Immunogen

Synthetic peptide within Human Olig2 aa 238-287 / 323.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000
IHC-F IHC-F: Immunohistochemistry-Frozen	1:500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:200
mIHC mIHC: Multiplex Immunohistochemical	1:1000-1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from SH-SY5Y(lane 2(20μg) , MCF-7(lane 3(20μg),Jurkat(lane 4(20μg)and Mouse Brain(lane 5(20μg) using Olig2 Antibody（HY-P86920）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/2000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

Background

Function：Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Functions together with ZNF488 to promote oligodendrocyte differentiation. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development

Subcellular Localization：Nucleus,Cytoplasm

Expression：
Tissue_Specificity: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable
Induction: By SHH. Also induced by NKX6-1 in the developing spinal cord, but not in the rostral hindbrain (By similarity)

Isoforms & Post-Translational Modification：Q13516: 323 amino acids, molecular weight 32385 Da.
Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation丨'Lys-48'-linked polyubiquitinated by NEURL3, leading to proteasomal degradation

Subunit：Interacts with NKX2-2

Synonyms

Basic domain helix loop helix protein class B 1 antibody; Basic helix loop helix protein class B 1 antibody; BHLHB antibody; bHLHB1 antibody; bHLHe19 antibody; Class B basic helix loop helix protein 1 antibody; Class B basic helix-loop-helix protein 1 antibody; class E basic helix loop helix protein 19 antibody; Class E basic helix-loop-helix protein 19 antibody; Human protein kinase C binding protein RACK17 antibody; Basic domain helix loop helix protein class B 1 antibody; Basic helix loop helix protein class B 1 antibody

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)