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Olig2 Antibody(YA6613)

(Synonyms: Basic domain helix loop helix protein class B 1 antibody; Basic helix loop helix protein class B 1 antibody; BHLHB antibody; bHLHB1 antibody; bHLHe19 antibody; Class B basic helix loop helix protein 1 antibody; Class B basic helix-loop-helix protein 1 antibody; class E basic helix loop helix protein 19 antibody; Class E basic helix-loop-helix protein 19 antibody; Human protein kinase C binding protein RACK17 antibody; Basic domain helix loop helix protein class B 1 antibody; Basic helix loop helix protein class B 1 antibody)
Customer Review

Based on 1 Customer Validation

Olig2 Antibody(YA6613) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Olig2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IF-Tissue, mIHC

  • Reactivity :

    Human, Mouse, Rat, Monkey, Pig

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Olig2 Antibody(YA6613) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
mIHC Info
mIHC: Multiplex Immunohistochemical
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:2000-1:5000 1:1000 1:500 1:200 1:1000-1:5000 1:200

Product Details

Description

Olig2 Antibody(YA6613) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Olig2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey, Pig
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 32 kDa
Immunogen

Synthetic peptide within Human Olig2 aa 238-287 / 323.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Olig2 Antibody(YA6613)
    Western blot analysis of extracts from SH-SY5Y(lane 2(20μg) , MCF-7(lane 3(20μg),Jurkat(lane 4(20μg)and Mouse Brain(lane 5(20μg) using Olig2 Antibody（HY-P86920）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/2000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    OLIG2 (oligodendrocyte transcription factor) is a basic helix-loop-helix transcription factor. The protein is of 329 amino acids in length, 32 kDa in size and contains one basic helix-loop-helix DNA-binding domain. It is one of the three members of the bHLH family. The other two members are OLIG1 and OLIG3. The expression of OLIG2 is mostly restricted in central nervous system, where it acts as both an anti-neurigenic and a neurigenic factor at different stages of development. OLIG2 is well known for determining motor neuron and oligodendrocyte differentiation, as well as its role in sustaining replication in early development. It is mainly involved in diseases such as brain tumor and Down syndrome.
    OLIG2 is highly expressed in glioblastoma, astrocytoma and oligodendrogliomas, and it serves as a diagnostic marker for glial tumors. OLIG2 also plays a critical role in gliomagenesis and tumor phenotype plasticity. Furthermore, OLIG2 expression has been shown to correlate with survival in patients with SHH-MBs[1][2].

  • Subcellular Localization

    Nucleus,Cytoplasm

  • Expression


    Tissue_Specificity: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable
    Induction: By SHH. Also induced by NKX6-1 in the developing spinal cord, but not in the rostral hindbrain (By similarity)

  • Isoforms & Post-Translational Modification

    Q13516: 323 amino acids, molecular weight 32385 Da.
    Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation丨'Lys-48'-linked polyubiquitinated by NEURL3, leading to proteasomal degradation

  • Subunit

    Interacts with NKX2-2

  • SwissProt ID

    Q13516

  • Gene ID
    10215 [NCBI]
  • Synonyms

    Basic domain helix loop helix protein class B 1 antibody; Basic helix loop helix protein class B 1 antibody; BHLHB antibody; bHLHB1 antibody; bHLHe19 antibody; Class B basic helix loop helix protein 1 antibody; Class B basic helix-loop-helix protein 1 antibody; class E basic helix loop helix protein 19 antibody; Class E basic helix-loop-helix protein 19 antibody; Human protein kinase C binding protein RACK17 antibody; Basic domain helix loop helix protein class B 1 antibody; Basic helix loop helix protein class B 1 antibody

References

[1]. Xu Z, et, al. OLIG2 Is a Determinant for the Relapse of MYC-Amplified Medulloblastoma. Clin Cancer Res. 2022 Oct 3;28(19):4278-4291. [Content Brief]

[2]. Xu R, et, al. OLIG2 Drives Abnormal Neurodevelopmental Phenotypes in Human iPSC-Based Organoid and Chimeric Mouse Models of Down Syndrome. Cell Stem Cell. 2019 Jun 6;24(6):908-926.e8. [Content Brief]

Olig2 Antibody(YA6613) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y(lane 2(20μg) , MCF-7(lane 3(20μg),Jurkat(lane 4(20μg)and Mouse Brain(lane 5(20μg) using Olig2 Antibody（HY-P86920）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/2000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Olig2 Antibody(YA6613) Product Image
    1/16
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Olig2 Antibody(YA6613) Product Image
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y(lane 2(20μg) , MCF-7(lane 3(20μg),Jurkat(lane 4(20μg)and Mouse Brain(lane 5(20μg) using Olig2 Antibody（HY-P86920）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/2000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Experimental Validation Results for Olig2 Antibody(YA6613)
  • Olig2 Antibody(YA6613) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from SH-SY5Y(lane 2(20μg) , MCF-7(lane 3(20μg),Jurkat(lane 4(20μg)and Mouse Brain(lane 5(20μg) using Olig2 Antibody（HY-P86920）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/2000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • Olig2 Antibody(YA6613) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Olig2 Antibody(YA6613) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Olig2 Antibody(YA6613) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Olig2 Antibody(YA6613) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Olig2 Antibody(YA6613) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Olig2 Antibody(YA6613) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Olig2 Antibody(YA6613) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using Olig2 Antibody (HY-P86920, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Olig2 Antibody(YA6613) Product Image WB
  • Olig2 Antibody(YA6613) Product Image IHC-P
  • Olig2 Antibody(YA6613) Product Image IHC-P
  • Olig2 Antibody(YA6613) Product Image IHC-P
  • Olig2 Antibody(YA6613) Product Image IHC-P
  • Olig2 Antibody(YA6613) Product Image IHC-P
  • Olig2 Antibody(YA6613) Product Image IHC-P
  • Olig2 Antibody(YA6613) Product Image IHC-P