CIRBP Antibody (YA4257)(PBS only)

Cat. No.: HY-P84560A: Data Sheet User Guide for Antibodies
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CIRBP Antibody (YA4257) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CIRBP.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CIRBP Antibody (YA4257) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CIRBP.

Host

Mouse

Molecular Weight

Predicted band size: 19 kDa;

Observed band size: 19 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q14011

Gene ID

1153 [NCBI]

Immunogen

Purified recombinant fragment of human CIRBP (AA: 1-90) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed

Subcellular Localization：Nucleus, nucleoplasm; Cytoplasm

Expression：
Tissue_specificity:general expression

Induction:By cold stress in response to DNA damage induced by UV irradiation or UV mimetic agents. Up-regulated by hypoxia

Isoforms & Post-Translational Modification：Q14011 has 3 isomers: Q14011-1: 18648 Da (predicted); Q14011-2: 28359 Da (predicted); Q14011-3: 26130 Da (predicted).
Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs (By similarity);Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation

Subunit：Interacts with EIF4G1. Associates with ribosomes

Synonyms

CIRP

Documentation

User Guide for Antibodies (1077 KB)