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ENO3 Antibody (YA2768)(PBS only)

(Synonyms: enolase 3; MSE; GSD13)

ENO3 Antibody (YA2768) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ENO3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
  • ENO3 Antibody (YA2768)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

ENO3 Antibody (YA2768) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ENO3.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Immunogen

A synthetic peptide of human ENO3

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
    Western blot analysis of extracts from Hela(lane 2(20ug)，NIH/3T3(lane 3(20ug) and C6(lane 4(20ug) using ENO3 Antibody (HY-P83023) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
    Immunocytochemistry analysis of Hela cells labeling ENO3 Antibody (HY-P83023) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ENO3 Antibody (HY-P83023) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
    Immunocytochemistry analysis of NIH/3T3 cells labeling ENO3 Antibody (HY-P83023) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ENO3 Antibody (HY-P83023) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    ENO3 is a Glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Appears to have a function in striated muscle development and regeneration

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons

  • Isoforms & Post-Translational Modification

    P13929 has 3 isomers: P13929-1: 46987 Da (predicted); P13929-2: 44115 Da (predicted); P13929-3: 42248 Da (predicted).

  • Subunit

    Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. Interacts with PNKD

  • SwissProt ID

    P13929

  • Gene ID
    2027 [NCBI]
  • Synonyms

    enolase 3; MSE; GSD13

  • Research Field

    Signal Transduction

ENO3 Antibody (YA2768)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug)，NIH/3T3(lane 3(20ug) and C6(lane 4(20ug) using ENO3 Antibody (HY-P83023) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ENO3 Antibody (HY-P83023) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ENO3 Antibody (HY-P83023) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH/3T3 cells labeling ENO3 Antibody (HY-P83023) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ENO3 Antibody (HY-P83023) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ENO3 Antibody (YA2768)(PBS only) Product Image
    1/16
  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
  • ENO3 Antibody (YA2768)(PBS only) Product Image
  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug)，NIH/3T3(lane 3(20ug) and C6(lane 4(20ug) using ENO3 Antibody (HY-P83023) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ENO3 Antibody (HY-P83023) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ENO3 Antibody (HY-P83023) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH/3T3 cells labeling ENO3 Antibody (HY-P83023) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ENO3 Antibody (HY-P83023) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
  • Experimental Validation Results for ENO3 Antibody (YA2768)(PBS only)
  • ENO3 Antibody (YA2768)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug)，NIH/3T3(lane 3(20ug) and C6(lane 4(20ug) using ENO3 Antibody (HY-P83023) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ENO3 Antibody (YA2768)(PBS only) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling ENO3 Antibody (HY-P83023) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ENO3 Antibody (HY-P83023) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ENO3 Antibody (YA2768)(PBS only) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH/3T3 cells labeling ENO3 Antibody (HY-P83023) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ENO3 Antibody (HY-P83023) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ENO3 Antibody (YA2768)(PBS only) Product Image WB
  • ENO3 Antibody (YA2768)(PBS only) Product Image ICC
  • ENO3 Antibody (YA2768)(PBS only) Product Image ICC