MEK4 Antibody (YA4967)
(Synonyms: MAP2K4; JNKK1; MEK4; MKK4; PRKMK4; SEK1; SERK1; SKK1; Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated pro)MEK4 Antibody (YA4967) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to MEK4.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in Ascitic fluid containing 0.03% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
MEK4 Antibody (YA4967) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to MEK4.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 42kDa;
Purified recombinant fragment of human MAP2K4 expressed in E. Coli. The exact sequence is proprietary to MCE.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in Ascitic fluid containing 0.03% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MEK4 dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to pro-inflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14
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Subcellular Localization
Cytoplasm; Nucleus
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Expression
Tissue_specificity:This gene is highly expressed in skeletal muscle and is also widely expressed in other tissues. -
Isoforms & Post-Translational Modification
P45985 has 2 isomers: P45985-1: 44288 Da (predicted); P45985-2: 45584 Da (predicted).
Activated by phosphorylation on Ser-257 and Thr-261 by MAP kinase kinase kinases (MAP3Ks) -
Subunit
Interacts with SPAG9 (By similarity). Interacts (via its D domain) with its substrates MAPK8/JNK1, MAPK9/JNK2, MAPK10/JNK3, MAPK11 and MAPK14. Interacts (via its DVD domain) with MAP3Ks activators like MAP3K1/MEKK1 and MAP3K11/MLK3. Interacts with ARRB1, ARRB2 and MAPK8IP3/JIP3
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SwissProt ID
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Synonyms
MAP2K4; JNKK1; MEK4; MKK4; PRKMK4; SEK1; SERK1; SKK1; Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated pro
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