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Phospho-Cyclin E1 (Thr395) Antibody

(Synonyms: CCNE1; CCNE; G1/S-specific cyclin-E1)
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Based on 1 Customer Validation

Phospho-Cyclin E1 (Thr395) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-Cyclin E1 (Thr395).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-Cyclin E1 (Thr395) Antibody
  • Phospho-Cyclin E1 (Thr395) Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Phospho-Cyclin E1 (Thr395) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-Cyclin E1 (Thr395).

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Species Reactivity Database

Entrez Gene: 898 Human

SwissProt: P24864 Human

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr395 of Human Cyclin E1.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3102977

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-Cyclin E1 (Thr395) Antibody
    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Phospho-Cyclin E1 Antibody (HY-P80807) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Cyclin E1 is essential for the control of the cell cycle at the G1/S (start) transition

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:It is highly expressed in the testes and placenta. Its expression level is lower in bronchial epithelial cells.

  • Isoforms & Post-Translational Modification

    P24864 has 3 isomers: P24864-1: 47077 Da (predicted); P24864-2: 41982 Da (predicted); P24864-3: 45150 Da (predicted).
    Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7;Ubiquitinated by UHRF2; appears to occur independently of phosphorylation

  • Subunit

    Interacts with CDK2 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex (PubMed:15660127). Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1 (PubMed:15178429). Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation (PubMed:21952639). Interacts with INCA1 (PubMed:21540187)

  • SwissProt ID

    P24864

  • Gene ID
    898 [NCBI]
  • Synonyms

    CCNE1; CCNE; G1/S-specific cyclin-E1

  • Research Field

    Cell Biology

Phospho-Cyclin E1 (Thr395) Antibody Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Phospho-Cyclin E1 Antibody (HY-P80807) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospho-Cyclin E1 (Thr395) Antibody Product Image
    1/16
  • Experimental Validation Results for Phospho-Cyclin E1 (Thr395) Antibody
  • Phospho-Cyclin E1 (Thr395) Antibody Product Image
  • Experimental Validation Results for Phospho-Cyclin E1 (Thr395) Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Phospho-Cyclin E1 Antibody (HY-P80807) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-Cyclin E1 (Thr395) Antibody
  • Phospho-Cyclin E1 (Thr395) Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Phospho-Cyclin E1 Antibody (HY-P80807) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospho-Cyclin E1 (Thr395) Antibody Product Image WB