Phospho-Cyclin E1 (Thr395) Antibody

Cat. No.: HY-P80807: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Phospho-Cyclin E1 (Thr395) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-Cyclin E1 (Thr395).

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Phospho-Cyclin E1 (Thr395) Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Phospho-Cyclin E1 (Thr395) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-Cyclin E1 (Thr395).

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 47 kDa;

Observed band size: 47 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P24864

Gene ID

898 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr395 of Human Cyclin E1.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Phospho-Cyclin E1 Antibody (HY-P80807) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Essential for the control of the cell cycle at the G1/S (start) transition

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:It is highly expressed in the testes and placenta. Its expression level is lower in bronchial epithelial cells.

Isoforms & Post-Translational Modification：P24864 has 3 isomers: P24864-1: 47077 Da (predicted); P24864-2: 41982 Da (predicted); P24864-3: 45150 Da (predicted).
Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7;Ubiquitinated by UHRF2; appears to occur independently of phosphorylation

Subunit：Interacts with CDK2 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex (PubMed:15660127). Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1 (PubMed:15178429). Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation (PubMed:21952639). Interacts with INCA1 (PubMed:21540187)

RRID

AB_3102977

Database

Entrez Gene: 898 Human

SwissProt: P24864 Human

OMIM: 123837 Human

Research Field

Cell Biology

Synonyms

CCNE1; CCNE; G1/S-specific cyclin-E1

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Phospho-Cyclin E1 (Thr395) Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-Cyclin E1 (Thr395) Antibody

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