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Fatty Acid Synthase Antibody (YA6054)

(Synonyms: FASN; FAS; Fatty acid synthase)
Customer Review

Based on 1 Customer Validation

Fatty Acid Synthase Antibody (YA6054) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fatty Acid Synthase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Fatty Acid Synthase Antibody (YA6054) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:2000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Fatty Acid Synthase Antibody (YA6054) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fatty Acid Synthase.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 273 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 273 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719132

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg), HEK293 (lane4, 20μg) and C2C12 (lane5, 20μg) using Fatty Acid Synthase Antibody (HY-P86362). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    Immunohistochemical analysis of paraffin-embedded human liver tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    Immunocytochemistry analysis of MCF7 cells labeling Fatty Acid Synthase with Fatty Acid Synthase Antibody (HY-P86362) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P86362) at 1:200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    Immunocytochemistry analysis of MCF7 cells labeling Fatty Acid Synthase with Fatty Acid Synthase Antibody (HY-P86362) at 1:400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P86362) at 1:400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Fatty Acid Synthase is a Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain; (Microbial infection) Fatty acid synthetase activity is required for SARS coronavirus-2/SARS-CoV-2 replication

  • Subcellular Localization

    Cytoplasm; Melanosome

  • Expression


    Tissue_specificity:It is widely distributed. It is significantly expressed in the brain, lungs, liver, and breast.

  • Subunit

    Homodimer which is arranged in a head to tail fashion (PubMed:17618296, PubMed:18022563, Ref.34). Interacts with CEACAM1; this interaction is insulin and phosphorylation-dependent; reduces fatty-acid synthase activity

  • SwissProt ID

    P49327

  • Gene ID
    2194 [NCBI]
  • Synonyms

    FASN; FAS; Fatty acid synthase

Fatty Acid Synthase Antibody (YA6054) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg), HEK293 (lane4, 20μg) and C2C12 (lane5, 20μg) using Fatty Acid Synthase Antibody (HY-P86362). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF7 cells labeling Fatty Acid Synthase with Fatty Acid Synthase Antibody (HY-P86362) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P86362) at 1:200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF7 cells labeling Fatty Acid Synthase with Fatty Acid Synthase Antibody (HY-P86362) at 1:400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P86362) at 1:400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Fatty Acid Synthase Antibody (YA6054) Product Image
    1/16
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Fatty Acid Synthase Antibody (YA6054) Product Image
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg), HEK293 (lane4, 20μg) and C2C12 (lane5, 20μg) using Fatty Acid Synthase Antibody (HY-P86362). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF7 cells labeling Fatty Acid Synthase with Fatty Acid Synthase Antibody (HY-P86362) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P86362) at 1:200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF7 cells labeling Fatty Acid Synthase with Fatty Acid Synthase Antibody (HY-P86362) at 1:400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P86362) at 1:400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA6054)
  • Fatty Acid Synthase Antibody (YA6054) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg), HEK293 (lane4, 20μg) and C2C12 (lane5, 20μg) using Fatty Acid Synthase Antibody (HY-P86362). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Fatty Acid Synthase Antibody (YA6054) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Fatty Acid Synthase Antibody (YA6054) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Fatty Acid Synthase Antibody (YA6054) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Fatty Acid Synthase Antibody (YA6054) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Fatty Acid Synthase Antibody (YA6054) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Fatty Acid Synthase Antibody (YA6054) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver tissue using Fatty Acid Synthase Antibody (YA6054). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86362, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Fatty Acid Synthase Antibody (YA6054) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF7 cells labeling Fatty Acid Synthase with Fatty Acid Synthase Antibody (HY-P86362) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P86362) at 1:200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Fatty Acid Synthase Antibody (YA6054) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF7 cells labeling Fatty Acid Synthase with Fatty Acid Synthase Antibody (HY-P86362) at 1:400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P86362) at 1:400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Fatty Acid Synthase Antibody (YA6054) Product Image WB
  • Fatty Acid Synthase Antibody (YA6054) Product Image IHC-P
  • Fatty Acid Synthase Antibody (YA6054) Product Image IHC-P
  • Fatty Acid Synthase Antibody (YA6054) Product Image IHC-P
  • Fatty Acid Synthase Antibody (YA6054) Product Image IHC-P
  • Fatty Acid Synthase Antibody (YA6054) Product Image IHC-P
  • Fatty Acid Synthase Antibody (YA6054) Product Image IHC-P
  • Fatty Acid Synthase Antibody (YA6054) Product Image ICC
  • Fatty Acid Synthase Antibody (YA6054) Product Image ICC