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ENPP1/PC1 Antibody (YA6891)

(Synonyms: Alkaline phosphodiesterase 1 antibody)
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Based on 1 Customer Validation

ENPP1/PC1 Antibody (YA6891) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ENPP1/PC1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ENPP1/PC1 Antibody (YA6891)
  • ENPP1/PC1 Antibody (YA6891) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:5000 1:1000

Product Details

Description

ENPP1/PC1 Antibody (YA6891) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ENPP1/PC1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 140 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 105 kDa
Immunogen

Recombinant protein within human ENPP1 aa 576-925.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ENPP1/PC1 Antibody (YA6891)
    Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HepG2 (lane 2(20μg)) and Human liver (lane 3(20μg)) using ENPP1/PC1 Antibody (HY-P87203) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    ENPP1/PC1 is a Nucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP and UTP to their corresponding monophosphates with release of pyrophosphate, as well as diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling; it is however unclear whether hedgehog inhibition is direct or indirect. Appears to modulate insulin sensitivity and function. Also involved in melanogenesis. Also able to hydrolyze 2',3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production. 2',3'-cGAMP degradation takes place in the lumen or extracellular space, and not in the cytosol where it is produced; the role of 2',3'-cGAMP hydrolysis is therefore unclear. Not able to hydrolyze the 2',3'-cGAMP linkage isomer 3'-3'-cGAMP[1][2][3][4][5][6][7][8].

  • Subcellular Localization

    Cell membrane,Basolateral cell membrane,Secreted

  • Expression


    Tissue_Specificity: Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis (PubMed:9344668). Expressed in melanocytes but not in keratinocytes (PubMed:28964717)

  • Isoforms & Post-Translational Modification

    P22413: 925 amino acids, molecular weight 104924 Da.

  • Subunit

    Homodimer (PubMed:28964717)

  • SwissProt ID

    P22413

  • Synonyms

    Alkaline phosphodiesterase 1 antibody

ENPP1/PC1 Antibody (YA6891) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HepG2 (lane 2(20μg)) and Human liver (lane 3(20μg)) using ENPP1/PC1 Antibody (HY-P87203) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • ENPP1/PC1 Antibody (YA6891) Product Image
    1/16
  • Experimental Validation Results for ENPP1/PC1 Antibody (YA6891)
  • ENPP1/PC1 Antibody (YA6891) Product Image
  • Experimental Validation Results for ENPP1/PC1 Antibody (YA6891)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HepG2 (lane 2(20μg)) and Human liver (lane 3(20μg)) using ENPP1/PC1 Antibody (HY-P87203) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ENPP1/PC1 Antibody (YA6891)
  • ENPP1/PC1 Antibody (YA6891) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HepG2 (lane 2(20μg)) and Human liver (lane 3(20μg)) using ENPP1/PC1 Antibody (HY-P87203) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • ENPP1/PC1 Antibody (YA6891) Product Image WB