ApoA-II Antibody (YA1459)

Cat. No.: HY-P81714: Data Sheet COA
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User Guide for Antibodies
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ApoA-II Antibody (YA1459) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ApoA-II.

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사이즈	재고	수량
10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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ApoA-II Antibody (YA1459) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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References

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ApoA-II Antibody (YA1459) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ApoA-II.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 11 kDa;

Observed band size: 11 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P02652

Gene ID

336 [NCBI]

Immunogen

A synthesized peptide derived from human Apolipoprotein A II aa50-100/100.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ICC

Immunocytochemistry analysis of A549 cells labeling ApoA-II with ApoA-II antibody (HY-P81714) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ApoA-II antibody (HY-P81714) at 1/50 dilution in BSA for Immunol Staining at 4℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of HepG2 cells labeling ApoA-II with ApoA-II antibody (HY-P81714) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ApoA-II antibody (HY-P81714) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism

Subcellular Localization：Secreted

Expression：
Tissue_specificity:Plasma; synthesized in the liver and intestine

Subunit：Monomer. Homodimer; disulfide-linked. Also forms a disulfide-linked heterodimer with APOD (PubMed:7918467). Interacts with NAXE and NDRG1

RRID

AB_3103821

Database

Entrez Gene: 336 Human ; 25649 Rat

SwissProt: P02652 Human ; P04638 Rat

OMIM: 107670 Human

Research Field

Cardiovascular

Synonyms

APO A2; Apo AII; APOA 2; ApoA II; APOA2; APOAII; Apolipoprotein A II

각종 서류

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/mda-mb-231-cell.html [Content Brief]

ApoA-II Antibody (YA1459) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:: ApoA-II Antibody (YA1459)
Cat. No.:: HY-P81714
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MedChemExpress Validation
Immunocytochemistry analysis of A549 cells labeling ApoA-II with ApoA-II antibody (HY-P81714) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ApoA-II antibody (HY-P81714) at 1/50 dilution in BSA for Immunol Staining at 4℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
1/16

MedChemExpress Validation
Immunocytochemistry analysis of HepG2 cells labeling ApoA-II with ApoA-II antibody (HY-P81714) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ApoA-II antibody (HY-P81714) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
1/16

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ApoA-II Antibody (YA1459)

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