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Glutamate Dehydrogenase 1 Antibody (YA3026)

(Synonyms: GDH; Gdh-X; GDH1; GLUD; Glud1; Glud1a; Glud1b; Gludl; MRG-2)
Customer Review

Based on 1 Customer Validation

Glutamate Dehydrogenase 1 Antibody (YA3026) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutamate Dehydrogenase 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Glutamate Dehydrogenase 1 Antibody (YA3026) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

Glutamate Dehydrogenase 1 Antibody (YA3026) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutamate Dehydrogenase 1.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 61 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human GLUD1 aa510-558/558.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105395

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
    Western blot analysis of extracts from HepG2 (lane 2(20μg), HepG2 (lane 3(40μg), using Glutamate Dehydrogenase 1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
    Immunocytochemistry analysis of HepG2 cells labeling Glutamate Dehydrogenase 1 with Glutamate Dehydrogenase 1 Antibody (HY-P83281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutamate Dehydrogenase 1 Antibody (HY-P83281) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
    Immunocytochemistry analysis of Hela cells labeling Glutamate Dehydrogenase 1 with Glutamate Dehydrogenase 1 Antibody (HY-P83281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutamate Dehydrogenase 1 Antibody (HY-P83281)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
    Immunohistochemical analysis of paraffin-embedded rat liver tissue using Glutamate Dehydrogenase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
    Immunohistochemical analysis of paraffin-embedded rat liver tissue using Glutamate Dehydrogenase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Glutamate Dehydrogenase 1 is a Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate[1][2][3][4][5][6].

  • Subcellular Localization

    Mitochondrion; Endoplasmic reticulum

  • Isoforms & Post-Translational Modification

    P00367 has 3 isomers: P00367-1: 61398 Da (predicted); P00367-2: 42950 Da (predicted); P00367-3: 46575 Da (predicted).
    ADP-ribosylated by SIRT4, leading to inactivate glutamate dehydrogenase activity (PubMed:16959573). Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer (PubMed:16023112)

  • Subunit

    Homohexamer (By similarity). Interacts with HADH; this interaction inhibits the activation of GLUD1 (By similarity)

  • SwissProt ID

    P00367

  • Gene ID
    2746 [NCBI]
  • Synonyms

    GDH; Gdh-X; GDH1; GLUD; Glud1; Glud1a; Glud1b; Gludl; MRG-2

  • Research Field

    Signal Transduction

Glutamate Dehydrogenase 1 Antibody (YA3026) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), HepG2 (lane 3(40μg), using Glutamate Dehydrogenase 1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Glutamate Dehydrogenase 1 with Glutamate Dehydrogenase 1 Antibody (HY-P83281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutamate Dehydrogenase 1 Antibody (HY-P83281) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Glutamate Dehydrogenase 1 with Glutamate Dehydrogenase 1 Antibody (HY-P83281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutamate Dehydrogenase 1 Antibody (HY-P83281)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using Glutamate Dehydrogenase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using Glutamate Dehydrogenase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Glutamate Dehydrogenase 1 Antibody (YA3026) Product Image
    1/16
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Glutamate Dehydrogenase 1 Antibody (YA3026) Product Image
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), HepG2 (lane 3(40μg), using Glutamate Dehydrogenase 1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Glutamate Dehydrogenase 1 with Glutamate Dehydrogenase 1 Antibody (HY-P83281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutamate Dehydrogenase 1 Antibody (HY-P83281) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Glutamate Dehydrogenase 1 with Glutamate Dehydrogenase 1 Antibody (HY-P83281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutamate Dehydrogenase 1 Antibody (HY-P83281)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using Glutamate Dehydrogenase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using Glutamate Dehydrogenase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Experimental Validation Results for Glutamate Dehydrogenase 1 Antibody (YA3026)
  • Glutamate Dehydrogenase 1 Antibody (YA3026) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2(20μg), HepG2 (lane 3(40μg), using Glutamate Dehydrogenase 1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Glutamate Dehydrogenase 1 Antibody (YA3026) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Glutamate Dehydrogenase 1 with Glutamate Dehydrogenase 1 Antibody (HY-P83281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutamate Dehydrogenase 1 Antibody (HY-P83281) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Glutamate Dehydrogenase 1 Antibody (YA3026) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Glutamate Dehydrogenase 1 with Glutamate Dehydrogenase 1 Antibody (HY-P83281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutamate Dehydrogenase 1 Antibody (HY-P83281)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Glutamate Dehydrogenase 1 Antibody (YA3026) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using Glutamate Dehydrogenase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Glutamate Dehydrogenase 1 Antibody (YA3026) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using Glutamate Dehydrogenase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Glutamate Dehydrogenase 1 Antibody (YA3026) Product Image WB
  • Glutamate Dehydrogenase 1 Antibody (YA3026) Product Image ICC
  • Glutamate Dehydrogenase 1 Antibody (YA3026) Product Image ICC
  • Glutamate Dehydrogenase 1 Antibody (YA3026) Product Image IHC-P
  • Glutamate Dehydrogenase 1 Antibody (YA3026) Product Image IHC-P