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Poliovirus Receptor/PVR Antibody (YA2556)

(Synonyms: PVR; PVS; Poliovirus receptor; Nectin-like protein 5; NECL-5; CD155)
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Based on 1 Customer Validation

Poliovirus Receptor/PVR Antibody (YA2556) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Poliovirus Receptor/PVR.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Poliovirus Receptor/PVR Antibody (YA2556)
  • Poliovirus Receptor/PVR Antibody (YA2556) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:40

Product Details

Description

Poliovirus Receptor/PVR Antibody (YA2556) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Poliovirus Receptor/PVR.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Species Reactivity Database

Entrez Gene: 5817 Human

SwissProt: P15151 Human

Immunogen

A synthesized peptide derived from human Poliovirus Receptor aa220-350/417.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104905

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Poliovirus Receptor/PVR Antibody (YA2556)
    Western blot analysis was performed on extracts from HT-1080 (lane 1, 15 μg), HUVEC (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), A549 (lane 4, 15 μg), and U-87MG (lane 5, 15 μg) using Poliovirus Receptor/PVR Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH , HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Poliovirus Receptor/PVR mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration; (Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport; (Microbial infection) Acts as a receptor for Pseudorabies virus; (Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Secreted; Secreted

  • Expression


    Induction:Transcriptionally regulated by SHH

  • Isoforms & Post-Translational Modification

    P15151 has 4 isomers: P15151-1: 45303 Da (predicted); P15151-2: 40125 Da (predicted); P15151-3: 39304 Da (predicted); P15151-4: 42864 Da (predicted).
    N-glycosylated. N-glycan at Asn-120: Hex5HexNAc4;Phosphorylated by Src kinases on tyrosine residues in the ITIM motif upon ligation. Interaction with TIGIT is required for Phosphorylation

  • Subunit

    Can form trans-heterodimers with NECTIN3. The extracellular domain interacts with VTN, CD226 and CD96. The cytoplasmic domain interacts with DYNLT1. Binds with high affinity to TIGIT

  • SwissProt ID

    P15151

  • Gene ID
    5817 [NCBI]
  • Synonyms

    PVR; PVS; Poliovirus receptor; Nectin-like protein 5; NECL-5; CD155

  • Research Field

    Immunology

Poliovirus Receptor/PVR Antibody (YA2556) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HT-1080 (lane 1, 15 μg), HUVEC (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), A549 (lane 4, 15 μg), and U-87MG (lane 5, 15 μg) using Poliovirus Receptor/PVR Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH , HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Poliovirus Receptor/PVR Antibody (YA2556) Product Image
    1/16
  • Experimental Validation Results for Poliovirus Receptor/PVR Antibody (YA2556)
  • Poliovirus Receptor/PVR Antibody (YA2556) Product Image
  • Experimental Validation Results for Poliovirus Receptor/PVR Antibody (YA2556)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HT-1080 (lane 1, 15 μg), HUVEC (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), A549 (lane 4, 15 μg), and U-87MG (lane 5, 15 μg) using Poliovirus Receptor/PVR Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH , HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Poliovirus Receptor/PVR Antibody (YA2556)
  • Poliovirus Receptor/PVR Antibody (YA2556) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HT-1080 (lane 1, 15 μg), HUVEC (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), A549 (lane 4, 15 μg), and U-87MG (lane 5, 15 μg) using Poliovirus Receptor/PVR Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH , HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Poliovirus Receptor/PVR Antibody (YA2556) Product Image WB