Poliovirus Receptor/PVR Antibody (YA2556)

Cat. No.: HY-P82811: Data Sheet COA
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Poliovirus Receptor/PVR Antibody (YA2556) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Poliovirus Receptor/PVR.

For research use only. We do not sell to patients.

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10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Poliovirus Receptor/PVR Antibody (YA2556) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Poliovirus Receptor/PVR Antibody (YA2556) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Poliovirus Receptor/PVR.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 45 kDa;

Observed band size: 70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P15151

Gene ID

5817 [NCBI]

Immunogen

A synthesized peptide derived from human Poliovirus Receptor aa220-350/417.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:40

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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Western blot analysis was performed on extracts from HT-1080 (lane 1, 15 μg), HUVEC (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), A549 (lane 4, 15 μg), and U-87MG (lane 5, 15 μg) using Poliovirus Receptor/PVR Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH , HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration; (Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport; (Microbial infection) Acts as a receptor for Pseudorabies virus; (Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Secreted; Secreted

Expression：
Induction:Transcriptionally regulated by SHH

Isoforms & Post-Translational Modification：P15151 has 4 isomers: P15151-1: 45303 Da (predicted); P15151-2: 40125 Da (predicted); P15151-3: 39304 Da (predicted); P15151-4: 42864 Da (predicted).
N-glycosylated. N-glycan at Asn-120: Hex5HexNAc4;Phosphorylated by Src kinases on tyrosine residues in the ITIM motif upon ligation. Interaction with TIGIT is required for Phosphorylation

Subunit：Can form trans-heterodimers with NECTIN3. The extracellular domain interacts with VTN, CD226 and CD96. The cytoplasmic domain interacts with DYNLT1. Binds with high affinity to TIGIT

RRID

AB_3104905

Database

Entrez Gene: 5817 Human

SwissProt: P15151 Human

OMIM: 173850 Human

Research Field

Immunology

Synonyms

PVR; PVS; Poliovirus receptor; Nectin-like protein 5; NECL-5; CD155

Documentation

Select Batch:

Data Sheet (232 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Poliovirus Receptor/PVR Antibody (YA2556) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Poliovirus Receptor/PVR Antibody (YA2556)

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