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Kaiso Antibody (YA2543)

(Synonyms: Kaiso; Zbtb33; ZNF Kaiso; ZNF348)
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Based on 1 Customer Validation

Kaiso Antibody (YA2543) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Kaiso.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Kaiso Antibody (YA2543)
  • Kaiso Antibody (YA2543) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Kaiso Antibody (YA2543) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Kaiso.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 85 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 74 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human KAISO aa620-672.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104043

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Kaiso Antibody (YA2543)
    Western blot analysis of extracts from HEK293T (lane 2, 20ug), MCF-7 (lane 3, 20ug), C6 (lane 4, 20ug) and HCT116 (lane 5, 20ug) using Kaiso Antibody (HY-P82798). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Kaiso transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1[1].

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:Expressed in vascular endothelium

    Induction:Induced in vascular endothelium by wounding. This effect is potentiated by prior laminar shear stress, which enhances wound closure

  • Subunit

    Self-associates. Interacts with CTNND1. Interacts with NCOR1. Interacts with KPNA2/RCH1. Interacts with CBFA2T3

  • SwissProt ID

    Q86T24

  • Gene ID
    10009 [NCBI]
  • Synonyms

    Kaiso; Zbtb33; ZNF Kaiso; ZNF348

  • Research Field

    Epigenetics and Nuclear Signaling

References

[1]. Barrett CW, et al. Kaiso directs the transcriptional corepressor MTG16 to the Kaiso binding site in target promoters. PLoS One. 2012;7(12):e51205. [Content Brief]

Kaiso Antibody (YA2543) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane 2, 20ug), MCF-7 (lane 3, 20ug), C6 (lane 4, 20ug) and HCT116 (lane 5, 20ug) using Kaiso Antibody (HY-P82798). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Kaiso Antibody (YA2543) Product Image
    1/16
  • Experimental Validation Results for Kaiso Antibody (YA2543)
  • Kaiso Antibody (YA2543) Product Image
  • Experimental Validation Results for Kaiso Antibody (YA2543)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane 2, 20ug), MCF-7 (lane 3, 20ug), C6 (lane 4, 20ug) and HCT116 (lane 5, 20ug) using Kaiso Antibody (HY-P82798). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Kaiso Antibody (YA2543)
  • Kaiso Antibody (YA2543) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T (lane 2, 20ug), MCF-7 (lane 3, 20ug), C6 (lane 4, 20ug) and HCT116 (lane 5, 20ug) using Kaiso Antibody (HY-P82798). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Kaiso Antibody (YA2543) Product Image WB