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QSOX1 Antibody (YA9792)

(Synonyms: QSOX1, FLJ34858, hQSOX, Human quiescin (Q6) mRNA, partial cds,)
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Based on 1 Customer Validation

QSOX1 Antibody (YA9792) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to QSOX1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for QSOX1 Antibody (YA9792)
  • QSOX1 Antibody (YA9792) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1000 1:100-200 1:50-100 1:20-50

Product Details

Description

QSOX1 Antibody (YA9792) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to QSOX1.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 55-95 kD Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Immunogen

Synthetic peptide of human Quiescin Q6

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for QSOX1 Antibody (YA9792)
    Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), HT-1080 (lane 3), U87 (lane 4), A549 (lane 5), SH-SY5Y (lane 6), and HeLa (lane 7, Negative) using QSOX1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    QSOX1 catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. Plays a role in disulfide bond formation in a variety of extracellular proteins. In fibroblasts, required for normal incorporation of laminin into the extracellular matrix, and thereby for normal cell-cell adhesion and cell migration[1][2][3][4][5][6].

  • Subcellular Localization

    Golgi apparatus membrane; Secreted

  • Expression


    Tissue_Specificity: Expressed in heart, placenta, lung, liver, skeletal muscle, pancreas and very weakly in brain and kidney.
    Induction: Induced in quiescent cells just as fibroblasts begin to leave the proliferative cycle and enter quiescence.

  • Isoforms & Post-Translational Modification

    QSOX1 has 2 isoforms, O00391-1: amino acid length is 747, molecular weight is 82578 Da (predicted); O00391-2: amino acid length is 604, molecular weight is 66861 Da (predicted).N-glycosylated (PubMed:17331072, PubMed:29757379). O-glycosylated on Thr and Ser residues (PubMed:29757379)

  • Subunit

    Monomer

  • SwissProt ID

    O00391

  • Synonyms

    QSOX1, FLJ34858, hQSOX, Human quiescin (Q6) mRNA, partial cds,

References

[1]. Chakravarthi S, et al. Intracellular catalysis of disulfide bond formation by the human sulfhydryl oxidase, QSOX1. Biochem J. 2007 Jun 15;404(3):403-11. [Content Brief]

[2]. Heckler EJ, et al. Human quiescin-sulfhydryl oxidase, QSOX1: probing internal redox steps by mutagenesis. Biochemistry. 2008 Apr 29;47(17):4955-63. [Content Brief]

[3]. Ilani T, et al. A secreted disulfide catalyst controls extracellular matrix composition and function. Science. 2013 Jul 5;341(6141):74-6. [Content Brief]

[4]. Grossman I, et al. An inhibitory antibody blocks the first step in the dithiol/disulfide relay mechanism of the enzyme QSOX1. J Mol Biol. 2013 Nov 15;425(22):4366-78. [Content Brief]

[5]. Javitt G, et al. cis-Proline mutants of quiescin sulfhydryl oxidase 1 with altered redox properties undermine extracellular matrix integrity and cell adhesion in fibroblast cultures. Protein Sci. 2019 Jan;28(1):228-238. [Content Brief]

[6]. Alon A, et al. The dynamic disulphide relay of quiescin sulphydryl oxidase. Nature. 2012 Aug 16;488(7411):414-8. [Content Brief]

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QSOX1 Antibody (YA9792) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), HT-1080 (lane 3), U87 (lane 4), A549 (lane 5), SH-SY5Y (lane 6), and HeLa (lane 7, Negative) using QSOX1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • QSOX1 Antibody (YA9792) Product Image
    1/16
  • Experimental Validation Results for QSOX1 Antibody (YA9792)
  • QSOX1 Antibody (YA9792) Product Image
  • Experimental Validation Results for QSOX1 Antibody (YA9792)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), HT-1080 (lane 3), U87 (lane 4), A549 (lane 5), SH-SY5Y (lane 6), and HeLa (lane 7, Negative) using QSOX1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for QSOX1 Antibody (YA9792)
  • QSOX1 Antibody (YA9792) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), HT-1080 (lane 3), U87 (lane 4), A549 (lane 5), SH-SY5Y (lane 6), and HeLa (lane 7, Negative) using QSOX1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • QSOX1 Antibody (YA9792) Product Image WB