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PDZK1 Antibody (YA1571)

(Synonyms: CAP70; CLAMP; PDZD1; NHERF3; NHERF-3)
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Based on 1 Customer Validation

PDZK1 Antibody (YA1571) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDZK1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PDZK1 Antibody (YA1571)
  • PDZK1 Antibody (YA1571) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

PDZK1 Antibody (YA1571) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDZK1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 57 kDa
Species Reactivity Database

Entrez Gene: 5174 Human

SwissProt: Q5T2W1 Human

Immunogen

A synthetic peptide of human PDZK1 aa 474-486.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103926

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PDZK1 Antibody (YA1571)
    Western blot analysis of extracts from HepG2 (lane 2, 20ug), Raji (lane 3, 20ug), HEK293 (lane 4, 20ug) and SiHa (lane 5, 20ug) using PDZK1 Antibody (HY-P81826). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PDZK1 is an A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with NHERF1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. Required for normal cell-surface expression of SCARB1. Plays a role in maintaining normal plasma cholesterol levels via its effects on SCARB1. Plays a role in the normal localization and function of the chloride-anion exchanger SLC26A6 to the plasma membrane in the brush border of the proximal tubule of the kidney. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function

  • Subcellular Localization

    Membrane; Peripheral membrane protein; Cell membrane

  • Expression


    Tissue_specificity:Expression is limited to epithelial cells. Expressed in the kidney (brush border of proximal tubule) , pancreas, liver, and small intestine. Expressed at a lower level in the adrenal cortex, testis and stomach. Overexpressed in breast, renal and lung carcinomas

  • Isoforms & Post-Translational Modification

    Q5T2W1 has 2 isomers: Q5T2W1-1: 57129 Da (predicted); Q5T2W1-2: 44665 Da (predicted).

  • Subunit

    Interacts with PDZK1IP1 and ABCC2. Interacts (via PDZ domains 1 and 3) with SCARB1 (C-terminal domain).

  • SwissProt ID

    Q5T2W1

  • Gene ID
    5174 [NCBI]
  • Synonyms

    CAP70; CLAMP; PDZD1; NHERF3; NHERF-3

  • Research Field

    Signal Transduction

PDZK1 Antibody (YA1571) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2, 20ug), Raji (lane 3, 20ug), HEK293 (lane 4, 20ug) and SiHa (lane 5, 20ug) using PDZK1 Antibody (HY-P81826). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PDZK1 Antibody (YA1571) Product Image
    1/16
  • Experimental Validation Results for PDZK1 Antibody (YA1571)
  • PDZK1 Antibody (YA1571) Product Image
  • Experimental Validation Results for PDZK1 Antibody (YA1571)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2, 20ug), Raji (lane 3, 20ug), HEK293 (lane 4, 20ug) and SiHa (lane 5, 20ug) using PDZK1 Antibody (HY-P81826). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PDZK1 Antibody (YA1571)
  • PDZK1 Antibody (YA1571) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2, 20ug), Raji (lane 3, 20ug), HEK293 (lane 4, 20ug) and SiHa (lane 5, 20ug) using PDZK1 Antibody (HY-P81826). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PDZK1 Antibody (YA1571) Product Image WB